XL08BH1 promoterCMVCopGFP-2A-Puro XL09BH1 promoterCMVPuro XL012BU6 promoterCMVNeo miRNA Lentiviral Vectors:MicroRNA (miRNA) are small, non-coding RNAs that play critical roles in regulating gene expression at the post-transcription level. PackGene’s miRNA lentiviral vectors are engineered to ove...
(wells indicated by black box in Panel A) using each lot and the standard deviation and coefficient of variation among wells from each lot were calculated (Panel B; n = 72 for each lot). As shown, average levels of GFP expression were comparable between lots, and the ...
(Han et al., 2009, 2011). For example, lentivirus is able to mediate ChR2-GFP expression in widespread and healthy neurons months after viral injections, and the expression of ChR2-GFP is well targeted to the plasma membrane (Fig. 1a and b). Detailed examination of the cell-type ...
Virus titers of 1 × 108 infectious particles per ml for both LV FXN and LV GFP were obtained in this way. 853 Cell culture Human primary fibroblasts from FRDA patients and controls (GM03665 and GM07492, respectively) were obtained from Coriell Cell Repository (Camden, New Jersey, USA), ...
A:If your lentivirus contains a reporter such as GFP, it is possible to measure the titer by infecting cells. We recommend using 293 or 293T cells which are easy to infect with lentivirus. Q: How can my lentiviral titer be improved?
ml by the infectious particles per ml values. This coefficient was used for pHR’SIN-cPPT-SFFV-FXN-WPRE titration, which was generated in parallel to pHR’SIN-cPPT-SFFV-eGFP-WPRE. Virus titers of 1 × 108infectious particles per ml for both LV FXN and LV GFP were obtained in this ...
CMV-controlled expression of the GFP marker gene from the pTK 303, pTK 399 and pTK 422 vectors is significantly lower than GFP expression from the traditional vector pTK 113. To improve transgene expression from a single LTR containing vector, a new shuttle vector, pTK469, was developed in ...
expression on cells, therefore, we tested various mammalian cells lines that were available to us and are known express hACE2. None of the cells transduced as efficiently as hACE2-293Ts; however, 293Ts produced decent amount of GFP + cells (Fig.2). Since the differences between hACE...