Neuron-specific expression of green fluorescent protein (GFP) with those promoters was examined in vivo by injecting the lentiviral vectors into the rat neostriatum, thalamus and neocortex. Among all the promoters, SYN promoter displayed the highest specificity for neuronal expression in all the ...
Among all the promoters, SYN promoter displayed the highest specificity for neuronal expression in all the regions examined (more than 96%). Although GFP production by the hybrid promoters was about 2-4 times larger than the non-enhanced promoters, the neuronal specificity was significantly ...
Define lentivirus. lentivirus synonyms, lentivirus pronunciation, lentivirus translation, English dictionary definition of lentivirus. n. pl. len·ti·vi·rus·es Any of a group of retroviruses of the genus Lentivirus that cause diseases with a long late
8.Neurons could survive in the culture for at least 8 weeks, during which the GFP was persistently expressed, indicating the lentivirus could efficiently and stably infect the neurons. 神经细胞至少能存活8周,在此期间GFP能持续表达,说明慢病毒能有效而稳定感染神经细胞。
found out that if they amplify CD9 expression in the LV producer cells, in the absence of VSVG protein on LVs, which is required for binding to LDL receptor of cells, the produced EV-based LVs containing viral proteins, transgene and the CD9 on their surface could minorly transduce the ...
During that period, the recombinant lentivirus was able to infect the neurons, while sustaining green fluorescence protein (GFP) expression. ER alpha recombinant lentivirus also infected the neurons at a MOT of 5. The ER alpha mRNA and protein expression levels were higher in the infected neurons...
Product Category: Pre-made recombinant lentivirus; LV-HB9-GFP; HB9-GFP Lentivirus Description: LV-HB9-GFP is pre-made lentivirus which expresses GFP under the mouse HB9 promoter. HB9 promoter is motor neuron specific promoter and will direct GFP expression exclusively in motor neurons. Ready ...
NR2B mRNA expression in the NR2B-RNAi-LV group was significantly lower than that in the control group and NC-GFP-LV group. This novel approach of silencing the NR2B gene in ACC neuron could potentially be used to alleviate pain-related aversion. 展开 关键词: RNA interference, NR2B, ACC, ...
transgene expression by integrating foreign genes into the host cell’s genome. The design of a recombinant lentivirus involves both substituting a portion of the lentivirus genome with a target gene and enhancing the virus’s capability to infect a broad range of cell types, including neurons,...
lobe. Protein was harvested from lungs 48 hr following infection with lentivirus. Western blotting using an anti-GFP antibody demonstrates GFP expression as a 37 kD band in the left lower lobe (lane 4), but not in other lobes (lanes 2, 3, 5) or in lungs of non-infected animals (lane...