dendritic cellsGenome-wide genetic approaches have proven useful for examining pathways of biological significance in model organisms such as and but similar techniques have proven difficult to apply to mammalian systems. Although manipulation of the murine genome has led to identificat...
RNA interference (RNAi) has recently emerged as a specific and efficient method to silence gene expression in mammalian cells either by transfection of sho... Rubinson,Douglas,A.,... - 《Nature Genetics》 被引量: 2868发表: 2003年 Biology of Oligodendrocyte and Myelin in the Mammalian Central...
In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a delta chain. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Distinct isoforms of this chain have different expression patterns ...
in titer, purity, viability, and consistency in producing your custom lentivirus. Recombinant lentivirus is derived from modifications of the HIV-1 genome and stands as the most widely used viral vector for effective gene delivery in mammalian cells. In contrast to plasmid DNA vectors that only ...
delivering your lentiviral construct into mammalian cells for expression of your protein of interest. Obtain detailed information on every step of the workflow, including preparation of the lentiviral construct, generation of the lentiviral stock, viral titering, transduction, and analysis of expr...
Lentiviruses are also used to delivery therapeutic drugs to the tumor microenvironment such as, Interferon (IFN) under the control of the Tie-2 promoter to transduce hematopoietic stem/progenitor cells (HSPC). IFN in appropriate doses is known to exert antiproliferative, angiostatic, immune cell-...
Finally, the recombinant lentiviral vector containing miR-124 (miR-124-LV) and the recombinant lentiviral vector containing the green fluorescent protein (GFP) reporter gene (NC-EGFP-LV) were obtained. The virus titer of the cells was determined using a gradient dilution method in a 24-well ...
(MOI) of 5. GFP expression in the infected cells was observed daily under an inverted fluorescence microscope. Five days after infection, ERα recombinant lentivirus-infected and -uninfected neuronal cells were collected, and total RNA and protein were extracted. ERα expression was examined by ...
expression on cells, therefore, we tested various mammalian cells lines that were available to us and are known express hACE2. None of the cells transduced as efficiently as hACE2-293Ts; however, 293Ts produced decent amount of GFP + cells (Fig.2). Since the differences between hACE...
SIN pRRLhCMVGFP lentivirus vectors were used to produce infectious particles by co-transfection of gag-pol pCMVΔR8.91 and pMD.G (for VSV-G envelope) into 293T cells. FIG. 2. Transduction of AEC with VSV-G pseudotyped lentivirus.