Explore our resources, tip and tricks, and troubleshooting advice for delivering your lentiviral construct into mammalian cells for expression of your protein of interest. Obtain detailed information on every s
Explore our resources, tip and tricks, and troubleshooting advice for delivering your lentiviral construct into mammalian cells for expression of your protein of interest. Obtain detailed information on every step of the workflow, including preparation of the lentiviral construc...
cells and the viral vector [105,106]. In this regard, poly-l-lysine, diethylaminoethyl (DEAE)-dextran,polybrene,protamine sulfate, or cationicliposomesare applied as positively-charged polycationic agents to diminish those repulsion forces [105,107–114]. Though utilizing polybrene inviral ...
Finally, the recombinant lentiviral vector containing miR-124 (miR-124-LV) and the recombinant lentiviral vector containing the green fluorescent protein (GFP) reporter gene (NC-EGFP-LV) were obtained. The virus titer of the cells was determined using a gradient dilution method in a 24-well ...
A image showing exceptional transfection efficiency of PolyJet™ reagent onHuman embryonic stem cells(hESCs).The hESCs grown in E8 medium on Geltrexvs(left panel, DIC imaging) was transfected with pEF1α-GFP. The GFP expression (right panel) was visualized by Nikon Eclipse Fluorescence microscope...
(MOI) of 5. GFP expression in the infected cells was observed daily under an inverted fluorescence microscope. Five days after infection, ERα recombinant lentivirus-infected and -uninfected neuronal cells were collected, and total RNA and protein were extracted. ERα expression was examined by ...
For example, we have studied gene-expression noise in HIV and its consequences, by utilizing lentiviral vectors expressing fluorescent reporter genes (e.g., green fluorescent protein, GFP) from the HIV-1 LTR promoter. These lentiviral vectors allowed us transduce Jurkat T cells (ATCC # TIB-152...
expression,FXNexpression in the human and mouse FRDA cells reached 96- and 210-fold, respectively, after infection, compared with uninfected FRDA cells 0.5- and 0.2-fold, respectively, compared with normal fibroblasts. Over time, this expression appeared to fall by ~50%, butFXNexpression ...
PepMute™ siRNA Transfection Reagent, formulated from simulation of virus cell penetrating peptides (CPPs), is a total novel siRNA delivery tool which provides more than 95% silencing efficiency at 1 nM siRNA in variety of mammalian cells. With our proprietary peptide simulation technology (PST...
SIN pRRLhCMVGFP lentivirus vectors were used to produce infectious particles by co-transfection of gag-pol pCMVΔR8.91 and pMD.G (for VSV-G envelope) into 293T cells. FIG. 2. Transduction of AEC with VSV-G pseudotyped lentivirus.