VSV-G pseudotyped retroviral vectors are more uniformly infectious over a broad range of tissues and species, compared to vector particles containing the amphotropic or xenotropic envelopes (16, 17). Retroviral vectors produced by transient transfection methods are generally higher in titer when using...
VSV-G-pseudotyped retroviral packaging through adenovirus-mediated inducible gene expression. 来自 Semantic Scholar 喜欢 0 阅读量: 48 作者:Y Yoshida,N Emi,H Hamada 摘要: It has been technically difficult to generate recombinant adenoviruses encoding genes for cytotoxic products such as vesicular ...
Particularly, to demonstrate the interaction between VSV G and Lgr4, the authors co-expressed the ectodomains of both proteins without their signal peptide (Zhang et al., 2017). Show abstract Reply to Rubinstein: Is Lgr4 essential for VSV- and VSV-G-pseudotyped lentiviral vector entry to ...
2. A Lentiviral Vector Pseudotyped with a Baboon Retrovirus Envelope Glycoprotein Outperforms VSV-G-LVs for Gene Transfer into Hematopoietic Stem Cells and Resting Lymphocytes Girard-Gagnepain A, Amirache F, Costa C, Levy C, Frecha C, Fusil F, et al. Baboon envelope pseudotyped LVs outperform ...
As lentivirus pseudotyped with VSV-G is capable of infecting human cells we strongly recommend BLAST searching the GN18 sequences to both verify that your sequence is unique and to determine if there are potential human targets.另外请问,这个GN18是干什么用的?
VSV-G Pseudotyped Lentiviral Vector Particles Produced in Human Cells Are Inactivated by Human Serum VSV-G pseudotyped lentiviral vector particles produced in human cells are inactivated by human serum. Mol Ther 2000;2:218-222. [PubMed: 10985952]... Nicholas,J.,DePolo,... - 《Molecular Ther...
In a previous study, we reported the prolonged cell surface retention of VSV-G replication-deficient pseudotyped lentivector prior to endocytic entry. However, the competing kinetics of cell surface versus dissociation, neutralization or direct transfer to other cells have received comparatively little ...
for efficient transduction of pigtailed macaque (Macaca nemestrina) long-term repopulating cells using VSV-G-pseudotyped HIV-based lentiviral vectors. Stable... GD Trobridge,BC Beard,C Gooch,... - 《Blood》 被引量: 88发表: 2008年 加载更多研究点推荐 Therapeutic efficiency ...
LoPRRNLo retrovirus was produced as a VSV-G protein pseudotype and used to infect primary human cells, resulting in the efficient formation of transformed cell lines. Subsequent introduction into the transformed cells of the lac repressor, expressed from a second retroviral vector, MSCV-In(S), ...
Pseudotransduction of hepatocytes by using concentrated pseudotyped vesicular stomatitis virus G glycoprotein (VSV-G)-Moloney murine leukemia virus-derived ... ML Liu,BL Winther,MA Kay - 《Journal of Virology》 被引量: 244发表: 1996年 A new system for stringent, high-titer vesicular stomatitis ...