Expression levels of MPT70 and ESAT-6 mRNA were analyzed prior to infection (week 0), at an early time point (week 4), and at a late time point (week 13). As expected, expression levels were below detection level prior to infection (Fig. 1a). At week 4, MPT70 expression was low...
To resemble the regulation of cathepsin L expression as closely as possible, we employed a knock-in approach in the murine cathepsin L gene to insert a point mutation into the M1- AUG (to TTC) followed by an intronic poly-(A)-signal which acts as a transcriptional stop signal and can ...