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Flow cytometry analysis was carried out with FlowJo_v10.8.1 software. Behavioral tests Anxious behavior and general locomotor activity were measured in the open field test, using a gray squared Plexiglas open box (50 cm2) (Panlab, S.L.). Briefly, mice were gently introduced in the open ...
Tightrope test Animals were placed on a 60 cm-long tightrope grasping the string with their forepaws according to a modified protocol from Doeppner et al.24. All animals were tested twice per time point. Maximum test time was 60 s, and results were scored from 0 (minimum) to 20 (maximum...
End stage was determined as the point when the animals could no longer turn themselves over within 10 s of being placed on their back, lost more than 20% of their peak weight, or had complete paralysis. Mice were provided with wet mashed food in their cages at the first sign of ...
(B) The ratios of the test cells (CD45.1/.2) to competitor cells (CD45.2) were measured in peripheral blood before and after 5-FU treatment. The 45.1/45.2 ratios were normalized to the day −3 time point. Error bars represent SD (n = 4). ∗p < 0.05. See also Figure S7. Dow...
(set to 1) for the low dose at 2 weeks for each reprogramming factor. Each point represents a single mouse. Center values are means and error bars are S.D. Statistical analyses were performed by one-way ANOVA with Tukey’s Multiple Comparison Test. *,p < 0.05; **,p < 0.01...
Recent advances in base editing have created an exciting opportunity to precisely correct disease-causing mutations. However, the large size of base editors and their inherited off-target activities pose challenges for in vivo base editing. Moreover, the
(Mean ± SEM: 509.2 ± 107.04, AAV1—101.9 ± 18.4,T-test,p < 0.001). AAV1*-infected neurons also show a tendency for higher expression within each cells (bottom)- AAV1* - 207.8 ± 5.9, AAV1—173.4 ± 12.04. Each data point represents one ...
The ability to genetically manipulate trigeminal ganglion (TG) neurons would be useful in the study of the craniofacial nervous system and latent alphaherpesvirus infections. We investigated adeno-associated virus (AAV) vectors for gene delivery to the T
an amber codon) of interest refers to the percentage of activity of a given expressed test marker (e.g., LacZ), that includes a selector codon, in a nucleic acid encoding the expressed test marker, in a translation system of interest, where the translation system of interest includes an ...