Virus overlay protein binding assay (VOPBA).ApteDeshpande, AnjaliPaingankar, MandarD. Gokhale, MangeshN. Deobagkar, Dileep
By using a virus overlay protein binding assay (VOPBA), in both nondenaturing and denaturing gel systems, a putative dengue virus serotype 1 binding ... C Thepparit,DR Smith - 《Journal of Virology》 被引量: 366发表: 2004年 Serotype-Specific Entry of Dengue Virus into Liver Cells: Identif...
bilayers. In all cholesterol-containing membranes and protein forms, distribution of cholesterol was enhanced next to the surface of the M protein as measured by the radial distribution function (Supplementary Fig.4a) indicating a favorable interaction of M proteins with cholesterol. Additionally, the ...
Overlay of the NTD (c), RdRp (d), PRNTase (e) domains and tetrameric P/VP35 protein (f) between EBOV L-VP35 and HPIV5 L-P complex shows a similar architecture, but with local differences. Extended Data Fig. 3 The paths for RNA synthesis within EBOV L-VP35 structure. (a) The...
In some experiments, the MoAb to HA was preincubated with Staph protein A in a 1:1 protein ratio at 37°C for 30 minutes. The complexed antibody-protein A unit was then used as the cross-linker of HA liposomes in the H2O2 measurement assay. Neuraminidase and Ganglioside Treatments of ...
Analysis of Binding Elements in the Human Immunodeficiency Virus Type 1 Genomic RNA and Nucleocapsid Protein Author links open overlay panelRobert D. Berkowitz, Stephen P. GoffShow more Add to Mendeley Share Cite https://doi.org/10.1006/viro.1994.1339Get rights and content...
1) virus overlay protein binding assay (VOPBA) 病毒铺覆蛋白结合试验(VOPBA) 2) Virus overlay protein binding assay 病毒铺覆蛋白结合试验 3) virus overlay protein blot assay(VOPBA) 病毒铺覆蛋白印迹技术(VOPBA) 4) VOPBA 病毒铺覆蛋白印迹技术 1. The cell membrane from permissive Vero cells ...
One of these studies demonstrated how using aptamers to bind the glycoprotein D (gD) protein of HSV-1 can inhibit viral entry into the host cells. The gD protein contains an ectodomain that is involved in the viral entry to the cells by coreceptor binding. This study involved two RNA ...
protein, as the transcript is co-terminal with the canonic transcript (and the shorter embedded). It is more likely that if this transcript encodes a protein after all (despite the low coding probability) that would be an N-terminally truncated version of the P protein and neither C nor C...
A research group of the National Institutes of Health (NIH) used this method to establish a high-throughput virus assay in 1536 well format to find inhibitors of the interaction between SARS-CoV-2 spike protein and ACE2 receptor. The viral spike protein attaches to the ACE2 receptor for cell...