The Protein Lipid Overlay (PLO) assay enables the identification of the lipid ligands with which lipid binding proteins interact. This assay also provides qualitative information on the relative affinity with which a protein binds to a lipid. In the PLO assay, serial dilutions of different lipids...
The protein-lipid overlay assay is an inexpensive, easy-to-implement, and high-throughput methodology that employs nitrocellulose membranes to immobilize lipids in order to rapid screen and identify pdoi:10.1007/978-1-4939-7201-2_13Tuo-Xian Tang...
The lipid overlay assay has been commonly used due to its convenience but it suffers from many drawbacks, including low sensitivity, poor reliability, and an inability to yield quantitative information (Dowler, Kular, & Alessi, 2002). Also, lipids are presented in a poorly defined, non...
Lipid transfer inhibitor protein (LTIP) exists in both active and inactive forms. Incubation (37°C) of plasma causes LTIP to transfer from a 470 kDa inactive complex to LDL where it is active. Here, we investigate the mechanisms underlying this movement. Inhibiting LCAT or cholesteryl ester ...
Lipid droplets (LDs) are crucial organelles for energy storage and lipid homeostasis. Autophagy of LDs is an important pathway for their catabolism, but the molecular mechanisms mediating LD degradation by selective autophagy (lipophagy) are unknown. Her
For successful infection of host cells and virion production, enveloped viruses, including Zika virus (ZIKV), extensively rely on cellular lipids. However, how virus protein–lipid interactions contribute to the viral life cycle remains unclear. Here, we
Samples were prepared as described using the lipid-DNA-method [9, 10]. Briefly, DNA mixtures containing reporter and prey (for PR slides) or reporter, prey and bait constructs (for PRB slides) in EC buffer supplemented with 0.2 M sucrose were treated for 5 minutes at RT with 1.5 μl En...
Methods for transfection include calcium phosphate (Chen and Okayama, 1987; Wigler et al., 1977), lipid-mediated (Felgner et al., 1989; Felgner and Ringold, 1989) and electroporation (Chu et al., 1987; Shigekawa and Dower, 198...
2. Protein and lipid as carriers 3. Techniques for manufacturing lipid-protein conjugate-based delivery systems 4. Characterization of the developed lipid-protein conjugate-based delivery systems 5. Applications of lipid-protein conjugate-based delivery systems 6. Lipid-protein conjugates-based products ...
A liquid phase of synapsin and lipid vesicles. Science 361, 604–607 (2018). Article CAS PubMed PubMed Central ADS Google Scholar Wang, Z. et al. Material properties of phase-separated TFEB condensates regulate the autophagy-lysosome pathway. J. Cell Biol. 221, e202112024 (2022). Article...