Mammalian expression systems, both transient and stable, were found to be the most favourable based on several criteria including receptor expression levels, correct folding and post translational processing, and capacity for purification of fully functional receptor. Transient expression using COS-1 cells...
enzyme promiscuity is also important for the evolution of novel functions [144]. Moreover, there is evidence that the interplay of protein mutational robustness, protein folding and environmental stress is an additional key factor that determines the evolution of new traits [...
However, enzyme-linked immunosorbent assays found no difference in binding between mTMV and wTMV. Functionalization of a nearby residue, tyrosine 139, with diazonium salt, also appears unaffected. Overall, this study highlights the necessity of standard workflows to quality-control viral stocks. We ...
However, this interaction was evaluated by bioinformatics analyses and not direct assays, hence it needs to be independently confirmed. Elevated Mirc1 (miR-17–92) cluster expression in CF sputum positively correlated with PEx, while there was a negative correlation with spirometric parameters [114]...
Despite some evident differences in gametocyte density and prevalence between adults and children, the data are not conclusive and are hard to compare due to lack of consistency in gametocytes estimation methods but also in infection models (skin or membrane feeding assays) [29, 89]. The non-...
bearing in mind, however, that lymphoblasts do not express some enzymatic activities, such as arylsulphatase A. Lysosomal enzyme assays are usually performed using synthetic (fluorimetric or colorimetric) substrates showing undetectable or very low enzyme activity in cell lines of affected individuals....
Various assays have been used to study specific toxicities. Direct cardiotoxicity was characterized by assessing the molecular changes in various cells. Selective organ damage was shown, where dermal fibroblasts [89] were previously shown not to be affected by exogenous toxicity, but damage was evident...
and hepatocytes produce IL-8 in response to B. henselae infection. We also investigated the role of IL-8 in B. henselae-induced endothelial cell proliferation and capillary tube formation. Both in vitro angiogenesis assays were IL-8 dependent. B. henselae-mediated inhibition of apoptosis, as ...
Principal conclusions are that standard assays and pathways are appropriate in most cases of possible Cushing syndrome. However, there are many pre-analytical and analytical challenges; therefore, there should be a low threshold to discuss alternative testing strategies with local experts if results are...