Trans-lentivirales Vektor System Trans-lentiviral vector systemJohn C KappesXiaoyun Wu
We report a lentiviral vector harboring the human β2-microglobulin promoter, with predominant expression in immune cells and minimal proximal enhancers to improve vector safety. This lentiviral vector efficiently transduces major dendritic cell subsets in vivo. With a mycobacterial immunogen, we observed ...
The application of novel emerging therapeutic strategies, such as lentiviral (LV) vectors, may help to stimulate peripheral nerve regeneration at a molecular level. In the experiments described here, we examined the effect of LV vector-mediated overexpression of nerve growth factor (NGF) and glial ...
Immunization of piglets with two doses of the lentiviral vector expressing JEV virus-like particles led to high titers of anti-JEV antibodies, that had efficient neutralizing activity regardless of the JEV genotype tested. Conclusions/Significance Immunization of pigs with the lentiviral vector ...
A vector capable of transducing non-dividing and/or slowly dividing cells is provided, wherein the vector is a lentiviral LTR-deleted vector. Also provided is a method for producing a protein of interest in a non-dividing or slowly dividing cell by transducing the cell with a lentiviral LTR-...
C. Optimizing the woodchuck hepatitis virus post-transcriptional regulatory element (WPRE) for safety and function in lentiviral vectors. Mol. Ther. 11, S322 (2005). Google Scholar Wang, L., Wang, H., Bell, P., McMenamin, D. & Wilson, J. M. Hepatic gene transfer in neonatal mice ...
In mice, we have previously reported that overexpression of WT and A30P α-synuclein by means of lentiviral vectors induces a similar rate in dopaminergic cell death and comparable α-synuclein pathology in a long-term study of 12 months [16]. In our hands, the overexpression levels rather ...
To develop a vaccine candidate against coronavirus disease 2019 (COVID-19), we generated a lentiviral vector (LV) eliciting neutralizing antibodies against the Spike glycoprotein of SARS-CoV-2. Systemic vaccination by this vector in mice, in which the expression of the SARS-CoV-2 receptor hACE...
Construction of stable packaging cell lines for clinical lentiviral vector production. Sci. Rep. 5, http://dx.doi.org/ 10.1038/srep09021.Sanber KS, Knight SB, Stephen SL, Bailey R, Escors D, Minshull J, Santilli G, Thrasher AJ, Collins MK, Takeuchi Y. 2015. Construction of stable ...
2. The recombinant lentiviral vector genome according to claim 1, wherein in the lentiviral 3′-LTR the promoter and the activator of the U3 region have been deleted, and wherein the polynucleotide encoding the prM and E proteins is placed under the control of a heterologous promoter. 3. Th...