Specific aminoacyl-tRNA synthetases can either promote or suppress tumourigenesis. Here we show that valine aminoacyl-tRNA synthetase (VARS) is a key player in the codon-biased translation reprogramming induced by resistance to targeted (MAPK) therapy in melanoma. The proteome rewiring in patient-...
Here we use a CRISPR–Cas9 screen to focus on genes that have been implicated in tRNA biogenesis, and identify a mechanism by which altered valine tRNA biogenesis enhances mitochondrial bioenergetics in T cell acute lymphoblastic leukaemia (T-ALL). Expression of valine aminoacyl tRNA synthetase is ...
Structural and mechanistic basis of pre- and posttransfer editing by leucyl-tRNA synthetase 2003, Molecular Cell Show abstract Structure-based analysis of protein-RNA interactions using the program ENTANGLE 2001, Journal of Molecular Biology Show abstract Aminoacyl-tRNA synthetases 2020, RNA Aminoacyl-tRN...
Extremely minor chemical changes such as the addition of a single methyl group to a binding partner can sometimes suffice to nearly eliminate binding; for example, the aminoacyl tRNA synthetase specific to the amino acid valine discriminates against the very similar side chain of the amino acid is...
1971: Fluorescence studies of the binding of valyl-tRNA synthetase and tryptamine to valine-specific tRNA. a possible role for tryptophan residues in the binding of aminoacyl-tRNA synthetases to tRNAs Febs Letters 17(1): 73-77 Favorova, O.O.; Sakharova, N.K.; Kazarinova, L.I.; Varsha...
Residues in a class I tRNA synthetase which determine selectivity of amino acid recognition in the context of tRNA. Certain aminoacyl-tRNA synthetases discriminate between closely similar amino acids by hydrolytic editing reactions in the presence of their cognate tRNA. ... E Schmidt,P Schimmel - ...
Expression of valine aminoacyl tRNA synthetase is transcriptionally upregulated by NOTCH1, a key oncogene in T-ALL, underlining a role for oncogenic transcriptional programs in coordinating tRNA supply and demand. Limiting valine bioavailability through restriction of dietary valine intake disrupted this ...
A method for constructing microorganism strains which produce amino acids comprising: combining in one bacterial genome (a) a mutation affecting the aminoacyl-tRNA synthetase corresponding to the selected amino acid, conferring cells auxotrophy which cannot be fully suppressed by addition of usual ...
A method for constructing microorganism strains which produce amino acids comprising: combining in one bacterial genome (a) a mutation affecting the aminoacyl-tRNA synthetase corresponding to the selected amino acid, conferring cells auxotrophy which cannot be fully suppressed by addition of usual ...
Other L-cysteine- and L-valine-activating enzymes present in crude extracts were identified as aminoacyl-tRNA synthetases which could be separated from ACV synthetase. The molecular mass of these enzymes is 140 kDa for L-valine ligase and 50 kDa for L-cysteine ligase. The dissociation constants ...