These reagents were used for qualitative comparative labeling of Escherichia coli valyl-tRNA synthetase (ValRS), an enzyme with Val/Ile editing activity, in order to identify the binding sites for l -valine or noncognate amino acids. Labeling of E.coli ValRS with the substrate analog valyl-...
建议收到该Valyl-tRNA synthetase 2, Mitochondrial (Putative) Antibody产品后,储存温度为-20°C。避免重复冻融其他 Abbexa总部位于英国剑桥的剑桥科学园,是一家专业的全球抗体供应商和蛋白质供应商,致力于为生命科学、药物开发和生物技术领域的科学家和研究者提供最佳的客户体验和高质量的产品。产品涵盖一抗、二抗、...
Region between residue 1214 to 1264 of Human Valyl-tRNA synthetase. 查看免疫原 偶联物 Unconjugated 形式 Liquid 浓度 1 mg/mL 纯化类型 Antigen affinity chromatography 保存液 phosphate/tris citrate, pH 7-8 内含物 0.09% sodium azide 保存条件 4° C 运输条件 Wet ice ...
Valyl-tRNA synthetaseHigh-motecular-mass complexHPLCA high-molecular-mass complex containing valyl-tRNA synthetase has been purified to homogeneity from rabbit liver. The molecular mass of the complex is about 800 kDa. The complex consists of four polypeptides of 130, 50, 40 and 30 kDa....
序比對的結果顯示酵母菌valyl-tRNA synthetase (ValRS)的N端也有一段帶正電荷的附屬區段,長約含97個胺基酸,這個序並未出現在大腸桿菌的同源酵素內.有趣的是:人ValRS 的N端除有似的帶正電荷附屬區段 (~胺基酸 201-300)外,它還多一段厭水性的附屬區段(~胺基酸 1-200),這個厭水性的區段已經被証實會與...
研究酵母菌Valyl-tRNA synthetase的tRNA結合功能;+B5636Study of the tRNA-Binding Activity of Yeast Valyl-tRNA Synthetase [[abstract]]比較原核及低等真核細胞(酵母菌)的tRNA synthetase可以發現:許多酵母菌的細胞質tRNA synthetase在其N或C端會多出一段多胜肽,稱為附屬區段,這些附屬區段存... 王健家,葉綠舒...
經過數十年探索,對於 aminoacyl-tRNA synthetases (aaRSs)的功能以及結構的理解已 有長足進展.相較之下,對於他們的演化路徑仍然相當模糊,尤其是具有雙重功能之基 因,例如 valyl-tRNA synthetase (ValRS).因此,在此我們著眼於酵母菌 ValRS 的演化情 形及其附加區段的功能.過去的研究指出酵母基因 VAS1 可轉譯出細胞...
Mechanism of molecular interactions for tRNAVal recognition by valyl-tRNA synthetase The molecular interactions between valyl-tRNA synthetase (ValRS) and tRNA(Val), with the C34-A35-C36 anticodon, from Thermus thermophilus were studied by c... FUKAI,S. - RNA 被引量: 89发表: 2003年 Incorrect ...
Valyl-tRNA synthetase (ValRS) strictly discriminates the cognate L-valine from the larger L-isoleucine and the isosteric L-threonine by the tRNA-dependent “double sieve” mechanism. In this study, we determined the 2.9 Å crystal structure of a complex of Thermus thermophilus ValRS, tRNAVal...
These data suggest that valyl-tRNA and isoleucyl-tRNA synthetases consist of two domains, each corresponding to the subunit of methionyl-tRNA synthetase. 展开 关键词: Valyl-tRNA synthetase Isoleucyl-tRNA synthetase Methionyl-tRNA synthetase Thermus thermophilus Amino acid composition Zinc content ...