Closer investigation revealed that a previously described structural motif of two arginine residues43, responsible for ATP fixation, seems to be involved in stabilizing the amino acid carboxyl group with its N-terminal arginine residue. The charged amino acid ligands in histidyl-tRNA synthetase (HisRS...
The aminoacyl‐tRNA synthetase carries out aneditingstep to ensure against misacylated tRNA being used in protein synthesis. Because the ribosome must treat all aminoacyl‐tRNAs as the same for making the peptide bond, any tRNA bearing the wrong amino acid would be used for protein synthesis, pos...
recent research has shed light on the profound significance of amino acids as bioactive molecules that play active roles in signaling pathways and metabolic regulation. mTOR, MYC and KRAS, which sense cellular amino acid levels and orchestrate these signals in a coordinated...
prolyl–tRNA synthetase (GluProRS) fusion protein was used. Consistent with observations of the crystal structures, both enzymes activated Aze (Fig.4a–c; no contamination with other amino acids was detected above 0.01% in our Aze stock.) However, at the same concentration of amino acid (1...
内容提示: Aminoacyl-tRNA synthetase inhibition activatesa pathway that branches from the canonicalamino acid response in mammalian cellsYeonjin Kim a,1 , Mark S. Sundrud b,1 , Changqian Zhou a , Maja Edenius a , Davide Zocco a , Kristen Powers a ,Miao Zhang a , Ralph Mazitschek...
1c). Universally across all six codons, the readthrough of tRNA LeuUAG decreased, highlighting the importance of its PTMs, which include the 5-oxyacetic acid uridine (cmo5U) modification28. As was observed with the wt tRNA mixture, the CUA codon was ambiguously read by non-cognate tRNAs ...
L. Amino acid sensing: architecture of mTORC1 on the lysosome surface. Curr. Biol. 30, R89–R91 (2020). Article CAS Google Scholar Han, J. M. et al. Leucyl-tRNA synthetase is an intracellular leucine sensor for the mTORC1-signaling pathway. Cell 149, 410–424 (2012). Article CAS ...
The results are consistent with the hypothesis that evolution of the full complement of aminoacyl-tRNA synthetases occurred prior to the divergence of prokaryotes and eukaryotes.关键词: aminoacyl-tRNA synthetase human nucleotide sequence amino acid sequence ...
This invention relates to an isolated nucleic acid fragment encoding an aminoacyl-tRNA synthetase. The invention also relates to the construction of a chimeric gene encod
Aminoacyl-tRNA synthetaseCatalysisConcertedDivalent metalsDynamicsEnzyme mechanismsInduced fitNucleophileRate-limiting stepSubstrate-assistedIn this chapter we consider the catalytic approaches used by aminoacyl-tRNA synthetase (AARS) enzymes to synthesize aminoacyl-tRNA from cognate amino acid and tRNA. This ...