The invention provides methods and compositions for a mutein aminoacyl-tRNA synthetase that preferentially charges a tRNA with a non-natural amino acid. Also provided are methods for incorporating the non-natural amino acid, para-methylazido-L-phenylalanine into a protein and further conjugating a ...
Signaling pathways that sense amino acid abundance are integral to tissue homeostasis and cellular defense. Our laboratory has previously shown that halofuginone (HF) inhibits the prolyl-tRNA synthetase catalytic activity of glutamyl-prolyl-tRNA synthetase (EPRS), thereby activating the amino acid response...
内容提示: Aminoacyl-tRNA synthetase inhibition activatesa pathway that branches from the canonicalamino acid response in mammalian cellsYeonjin Kim a,1 , Mark S. Sundrud b,1 , Changqian Zhou a , Maja Edenius a , Davide Zocco a , Kristen Powers a ,Miao Zhang a , Ralph Mazitschek...
Closer investigation revealed that a previously described structural motif of two arginine residues43, responsible for ATP fixation, seems to be involved in stabilizing the amino acid carboxyl group with its N-terminal arginine residue. The charged amino acid ligands in histidyl-tRNA synthetase (HisRS...
Aminoacyl-tRNA synthetases are a family of enzymes responsible for ensuring the accuracy of the genetic code by specifically attaching a particular amino acid to their cognate tRNA substrates. Through primary sequence alignments, prolyl-tRNA synthetases (ProRSs) have been divided into two phylogenetically...
rat myocardial aminoacyl-tRNA synthetase activity, transfer of amino acids from aminoacyl-tRNA into protein, or the incorporation of amino acid into protein ... KEITHGIBSON,PETERHARRIS - 《Cardiovascular Research》 被引量: 0发表: 1974年 Purification and properties of leucyl-tRNA-synthetase from the...
amino acid n. Any of various compounds containing an amino group (NH2), a carboxylic acid group (COOH), and a distinctive side chain, especially any of the 20 amino acids that link together to form proteins. Some amino acids (called nonessential) can be synthesized in the human body, whi...
THE correct attachment of amino acids to their corresponding (cognate) transfer RNA catalysed by aminoacyl-tRNA synthetases is a key factor in ensuring the... M Jahn,MJ Rogers,D S?Ll - 《Nature》 被引量: 228发表: 1991年 Amino Acid Discrimination by a Class I Aminoacyl-tRNA Synthetase Speci...
aminoacidaemia aminoacidemia aminoacidemia aminoacidopathy aminoacidosis Aminoacids Aminoacids Aminoacids Aminoacids aminoaciduria Aminoaciduria, renal Aminoaciduria, renal Aminoaciduria, renal aminoacyl aminoacyl adenylate Aminoacyl site Aminoacyl site Aminoacyl site aminoacyl tRNA Aminoacyl tRNA synthetase Aminoa...
The aminoacyl-tRNA synthetases are an ancient group of enzymes that catalyze the covalent attachment of an amino acid to its cognate transfer RNA. The ques... PJ Beuning,K Musier-Forsyth - 《Biopolymers》 被引量: 261发表: 1999年 Hydrolytic editing by a class II aminoacyl-tRNA synthetase. Ed...