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The intraluminal space of the endosome and lysosome is acidified by vacuolar-type [H.sup.+] ATPase (V-ATPase) at the endosomal membrane. Maoto, a Traditional Japanese Herbal Medicine, Inhibits Uncoating of Influenza Virus Mutating subunits of the vacuolar proton-translocating ATPase (v-ATPase) ...
Expression of dominant-negative (GDP-bound form) Rab7 inhibited lysosome trafficking in wild-type cells. Furthermore,a3 directly interacted with the GDP-bound forms of Rab7 and Rab27A. These findings reveal a novel role for the proton pump V-ATPase in secretory lysosome trafficking and an ...
The lysosome degrades and recycles macromolecules, signals to the cytosol and nucleus, and is implicated in many diseases. Here, we describe a method for the rapid isolation of mammalian lysosomes and use it to quantitatively profile lysosomal metabolites under various cell states. Under nutrient rep...
In contrast, BafilomycinA1 prevents autophagosome-lysosome fusion in these cells, and this defect is phenocopied by depletion of the Capump SERCA, a secondary target of this drug. Moreover, activation of SERCA promotes fusion in a BafilomycinA1-sensitive manner. Collectively, our results indicate ...
近日,有研究人员对此进行了研究。相关研究成果发表在《Nature》上,文章标题为:“Low-dose metformin targets the lysosome–AMPK pathway through PEN2”。 研究人员发现临床相关浓度的二甲双胍抑制了溶酶体质子泵v-ATPase,这是葡萄糖饥饿后AMPK激活的中心节点。研究人员合成了一种光活性二甲双胍探针,并确定了PEN2, ...
V-ATPase is not required for autophagosome–lysosome fusion BafA1, a chemical inhibitor of the V-ATPase, is commonly used to block autophagosome–lysosome fusion in mammalian cell culture studies, and causes an accumulation of autophagosomes upon autophagy induction14,24. This phenomenon is reversible...
Other V-ATPase–dependent mechanisms that can influence lysosomal acidification include changes to the coupling efficiency of the enzyme and changes in the density of V-ATPase complexes per lysosome (1, 4). Therefore, it was important to test whether the reduced response to amino acid starvation ...