Autophagosome-lysosome fusion is independent of V-ATPase-mediated acidification. Nat Commun 2015;6:7007.Mauvezin C, Nagy P, Juhasz G, Neufeld TP. Autophagosome-lysosome fusion is independent of V-ATPase-mediated acidification. Nature communications 2015; 6:7007. 211. Xie R, Nguyen S, McKeehan ...
The ATP-dependent proton pump V-ATPase ensures low intralysosomal pH, which is essential for lysosomal hydrolase activity. Based on studies with the V-ATPase inhibitor BafilomycinA1, lysosomal acidification is also thought to be required for fusion with incoming vesicles from the autophagic and endocy...
The ATP-dependent proton pump V-ATPase ensures low intralysosomal pH, which is essential for lysosomal hydrolase activity. Based on studies with the V-ATPase inhibitor BafilomycinA1, lysosomal acidification is also thought to be required for fusion with incoming vesicles from the autophagic and endocy...
This acidic characteristic has been attributed to the activity of vacuolar-type H+-ATPase (V-ATPase), which continuously pumps protons into lysosomes in an energy-dependent manner. Thus, it is understandable that alkalinity hinders lysosomal ability to degrade substrates. Additionally, live cell ...
However, the same study also showed that V-ATPase inhibition did not affect the efflux of most essential amino acids, which was instead found to be regulated by mTORC1 activity in an SLC38A9-dependent manner53. Hence, catabolite efflux from lysosomes may be subjected to several regulatory ...
3). The phagosome interior becomes more acidic as a result of the gradual accumulation of active V-ATPase proteins on its membrane. The V-ATPase is an enzyme that moves protons (H+) into the interior of the phagosome, using ATP from the cytoplasm as an energy source (Marshansky and ...
Medical Encyclopedia Wikipedia Related to lysosome:primary lysosome ly·so·some (lī′sə-sōm′) n. A membrane-bound organelle in the cytoplasm of most cells containing various hydrolytic enzymes that function in intracellular digestion.
This review will discuss the functions of lysosomes and lysosomal-related organelles, with particular emphasis on the major proton pump, the vacuolar-type proton ATPase (V-ATPase), which is responsible for luminal acidification. 展开 关键词: Animals Lung Spermatozoa Acrosome Kidney Lysosomes Vacuoles ...
trans-Golgi network protein 2; TIRF: total internal reflection fluorescence; TMEM106B: transmembrane protein 106B; TOR: target of rapamycin; TRPM2: transient receptor potential cation channel subfamily M member 2; V-ATPase: vacuolar-type proton-translocating ATPase; VPS35: VPS35 retromer complex co...
contains reactive oxygen species and reactive nitrogen species as well as hydrolytic enzymes that are essential for its degradative function. Inside the phagolysosome is an acidic environment because of its proton pumps (v-ATPases) that transport H+across the membrane. Its acidity further aids in ...