By utilizing these results, many ongoing fluorescence microscopy studies in living cells, which are currently performed with a diffraction-limited confocal microscope, can be carried out with a substantially improved spatial resolution in all directions by means of STED microscopy. Altogether, this pave...
CRISPR imaging data were acquired on a Nikon Ti-E inverted wide-field fluorescence microscope equipped with a ×100 NA 1.40 PlanApo oil immersion objective, an LED light source (Excelitas X-Cite XLED1), an sCMOS camera (Hamamatsu Flash 4.0), and a motorized stage (ASI) with stage incubato...
Milk samples with added whey (from 0 to 100%) were prepared, simulating different levels of fraudulent adulteration. All analyses were carried out by direct inspection at the light microscope after depositing drops from each sample on a microscope slide and drying them at room temperature. No ...
The formation of macromolecular complexes can be measured by detection of changes in rotational mobility using time-resolved fluorescence anisotropy. However, this method is limited to relatively small molecules (~0.1–30 kDa), excluding the majority
Cardiac fibrosis after myocardial infarction (MI) has been considered an important part of cardiac pathological remodeling. Immune cells, especially macrophages, are thought to be involved in the process of fibrosis and constitute a niche with fibroblast
However, they serve as a complementary insect model to Drosophila for studying functional male fertility. In this study, we used a comparative method combining taxonomic, phenotypic data and gene expression to identify candidate genes that could play a significant role in spermatogenesis in hymenoptera...
We also performed a secondary HTS for diploid proliferative growth using the same chemicals, to distinguish compounds solely affecting meiosis from compounds affecting both meiosis and mitosis. For the proliferative growth assay, cells were chronically exposed throughout the assay without chemical washout....
It is less than the mean collective growth rate with 1 bud, which represents the procession towards mitosis, but it is greater than the mean single-cell growth rate, which represents the sole growth of the mother cell. There were three cases where a second daughter was able to emerge near...
(dinaciclib, paclitaxel, staurosporine, or vincristine) at nine doses spanning four orders of magnitude. The YOYO-1 vital dye was added to the medium to detect dead and dying cells, and imaging was performed on a microscope with an environmental control chamber equipped to handle microtiter ...
On the contrary, small interfering RNA (siRNA) is a kind of dsRNA molecule functioning as a RNAi tool used commonly to silence cancer-related gene expressions including polo-like kinase 1 (PLK1) and B-cell lymphoma-2 (Bcl-2). The impairment of mitosis machinery and the antiapoptotic cellular...