For example, cell images under phase contrast microscope contain halos and shade-offs. The imaging model can be generally formulated as (6.11)g=P⊛f+C where f represents the ideal image (the artifact-free image to be restored), g represents the observed images that must be dealt with ...
The progress of demembranation was followed at 1 min intervals by staining 1 μl of the sperm mix with 4 μl of 1 μg/ml Hoechst 33342 and observing the sperm under a microscope until all the sperm heads stained (about 10 min). The reaction was quenched by addition of 9.5 ml XN ...
Zebrafish were maintained at 28.5 1C under a reproduction regime (14-h light/10-h dark cycle). All embryos were collected after natural spawning and staged in terms of previous report.30 Semi-quantitative PCR and real-time quantitative PCR. Total RNAs were extracted by RNeasy plus mini kit ...
3 leading to a mitotic arrest that will eventually trigger cell death4 by mechanisms that are not well under- stood.5 The mitotic block observed following taxane exposure derives from activation of the spindle assembly checkpoint (SAC),6 which controls metaphase–anaphase transition by ensuring that...
Currently mitosis counting is done manually by a pathologist looking at multiple high power fields on a glass slide under a microscope, an extremely laborious and time consuming process. The development of computerized systems for automated detection of mitotic nuclei, while highly desirable, is ...
Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution License ( https://creativecommons.org/licenses/by/2.0 ), which permits unrestricted use, distribution, and reproduction in an...
o If you were looking under a microscope at a cell, what would tell you that it is in Interphase (i.e., what can be seen and what is not seen that would indicate mitosis is not yet occurring)? Prophase Next model Prophase. The nuclear membrane is starting to break down and the ...
(470 nm ex., 485–650 nm em.) in a microliter cuvette. The dynabeads were then mixed in the lysate for 1 h at 4 °C under constant, mild agitation. Beads were then magnetically separated, the fluorescent emission of the remaining lysate was measured again with the same ...
Briefly, dried peptides were reconstituted in 0.1% trifluoroacetic acid solution and then loaded onto a high-pH, reversed-phase fractionation spin column. Peptides were bound to the hydrophobic resin under aqueous conditions and desalted by washing the column with water. Subsequently, bound peptides...
In cell suspensions under agitation this is not a source of error if the aliquot can be considered to be representative. Presumably the concern would be with the whole cell population, as indeed it can be in all cases if the frame of reference is the whole population, the sampled cells ...