In optical setups involving a high-NA microscope objective where the paraxial approximation does not hold, we expect the ASM to perform better than in our test setup. Figure 4 Convergence of the feedback procedure using the FFT and the ASM, with and without pixel crosstalk modelling. The ...
Light scattered back from the scene is focused on the SPAD (Micro Photon Devices PD-100-CTD) using a microscope objective. A PicoQuant PicoHarp 300 module is used for time stamping, histogram accumulation, and synchronization of laser and detector. We use a pulse rate of 5 MHz, resulting ...
Results of the study show that DIC is a powerful tool in performing displacement/strain measurements on the microscale using a light microscope provided that an adequate correction is employed for image distortion. 展开 关键词: Practical, Experimental/ aberrations displacement measurement lenses optical ...
As such, if we can identify more macro trends (week to week), perhaps we can then apply that knowledge to when we see those trends forming within shorter periods (hour to hour or day to day), the same way we can look at a leaf in our hand, then put it under a microscope and lo...
Figure 1. 2p-MuST Microscope with Remote Scanning (A) Sensitivity S to evaluate power penalty in the microscope design for 2p excitation. Analysis for one-pulse-per-voxel acquisition, with DL (0.5 μm, light orange), 5 μm TeFo (red), and 10 μm TeFo (orange) PSFs with 5 μm sa...
Practical Tips for Fluorescence Imaging Using the EVOS FL Auto Cell Imaging System The EVOS FL Auto Imaging System is an automated imaging platform capable of live cell imaging, area scanning, image stitching, and time-lapse imaging. The microscope supports any type of culture dish. In this ...
Fig. 5.TEM images of (a)purecurcumin Ag NPscolloid(b) after addition of TNT [10−9M], (c) addition of TNT [10−6M] andoptical microscopeimage after addition of TNT [10−3M]. It is observed from the image that the pure CAgP are highly dispersed and found to be of average si...
By combining this system with a shear-force microscope, semiconducting carbon nanotubes are imaged with a spatial resolution of 60nm. Our results show that tip-enhanced broadband CARS image exhibits a positive contrast even though the enhancement effect has not been fully optimized. Analyses of ...
Microscope images (see Supplementary Fig. S6a and b) of these beads reveal the presence of some non-spherical particles, the shape of which has an influence on their scattering pattern. Supplementary Fig. S6c and d shows the performance of Model 2 for all glass beads except the Guyson ...
A scanning electron microscope (SEM, QUANTA 200) obtained from Thermo Fisher Scientific in USA was used to observe the microstructure of the microspheres. ImageJ software was used to calculate the average value of microspheres (n = 30) as their mean particle size. ...