Here, we examined the formation and function of meiotic midbodies (mMB) and mMB remnants using mouse oocytes and demonstrate that mMBs have a specialized cap structure that is orientated toward polar bodies. We show that that mMBs are translationally active, and that mMB caps are required to ...
The invention of the microscope led to the explosive development of biological research, including brain research. After Ernst Abbe’s diffraction limit (λ/2, where λ is the wavelength of light) was published in 1873, Stefan W. Hell developed stimulated emission depletion microscopy, which overca...
(180 μl), transferred to a 96-well plate or a 96-well glass-bottomed plate (AGC Techno Glass), and observed under a BZ-8000 all-in-one type microscope (Keyence) with a 20×, 0.75 NA objective lens under fixed conditions (sensitivity ISO 400, exposure 1/10 s for green fluorescence;...
Capricorn is the practical idealist and has the determination to surmount all obstacles en route to the peak of experience. They have the power to manifest based on their use of the resources available and their understanding of the underlying structure necessary for achievement. There are rules to...
Samples were then stained with 2% uranyl acetate and were observed using a Tecnai T12 electron microscope at an accelerating voltage of 100 kV. Images were taken digitally with a Teitz TCL camera system. Pili conductivity. As previously described (11), for conductivity mea- surements, pili ...
Therefore, although EST is a prominent inhibitor of Nobo, a practical compound that can be utilized as an actual insecticide must display no estrogenic activity. To consider this problem, it is important to note a difference in the EST-recognition patterns between DmNobo and the mammalian ...
The analysis of the MEs of wild-type and mutant cells in the electron microscope revealed that the ESCRT-containing electron-dense microdomains of ILV formation at the limiting membrane are elongated, indicating a change in ESCRT activity. Since lgd mutants can be rescued to normal adult flies ...
a platform when the platform was not visible (“hidden training”) (Fig.1a). One day after the sixth session of hidden training, a probe test (Probe test 1) was conducted without a platform to determine whether mice had learned the location of the platform using extra-maze cues (Fig.1a...
A practical method for grading the cognitive state of patients for the clinician. J. Psychiatr. Res. 12, 189–198 (1975). Article CAS Google Scholar Huntington Study Group Unified Huntington’s Disease Rating Scale: reliability and consistency.Mov. Disord. 11, 136–142 (1996). Article ...
1e). To generate mutations in this complex locus, the P[GAL4]4C was excised, using the standard genetic method of excision36 resulting in a small 632 bp deletion (F4) (shortly named: Del) (Fig. 1a and Supplementary Fig. 1a), which completely removed the snoRNA:Ψ28S-1153 as well...