the more the image is degraded by out-of-focus information. This creates a significant need to generate axial contrast whenever high lateral resolution is required. One strategy for generating contrast is to measure or model theoptical propertiesof the microscope and to use that model to algorithmi...
A practical guide for the easy implementation of a Fourier light-field microscope is reported. The Fourier light-field concept applied to microscopy allows the capture in real time of a series of 2D orthographic images of microscopic thick dynamic samples. Such perspective images contain spatial and...
of thetelescopedramatically broadened exploration of the universe, so too the invention of themicroscopeopened the intricate world of thecell. The analysis of the frequencies of light emitted and absorbed byatomswas a principalimpetusfor the development ofquantum mechanics. Atomic and molecular ...
image contrast is generated based on scattering and absorbing of the incident light. However, most biological specimens are inherently weakly absorptive and scattering, thus are almost transparent in a conventional bright field microscope. A number of approaches for increasing...
TheCalibration and Performance extensionintroduces specifications for the capture of metrics required for microscope calibration and quantitative instrument performance assessment (Fig.4, maroon elements; Supplementary Figs. 1 and 4). Although it would be impracticable for the current version of the specific...
Nevertheless, diffraction is often the limiting factor in the ability of a microscope to resolve the fine details of objects. The late 19th-century French painter Georges Seurat created a new technique, known as pointillism, based on diffraction effects. His paintings consist of thousands of ...
(Λ = 0.28 µm,h> 50 nm). Here, the 3D micro-device proved to be capable of enhancing lateral resolution beyond the limits achievable with conventional lenses or microscope objectives when used in air. Simultaneously, it maintained the high axial resolution characteristic of Mirau-type ...
Imagine if it were possible to create 3D objects in the palm of your hand within seconds using only a single photonic chip. Although 3D printing has revolutionized the way we create in nearly every aspect of modern society, current 3D printers rely on la
230 In this approach, a laser source fires two or more incoming photons, which are focused by the microscope and which penetrate deeply into biological specimens, more deeply than visible light. This high-energy level excites fluorophores that emit a single photon in the visible spectrum. This ...
Light sheet microscopes reduce phototoxicity and background and improve imaging speed compared to widefield and confocal microscopes. However, when equipped with Gaussian beams, the axial resolving power of a light sheet microscope and the observable fie