A good physics textbook should be consulted if a more detailed explanation of image production by lenses is required. A light microscope essentially consists of three components, the eyepiece, the objective and an illumination source (condenser). The last two have greatest influence on image quality...
Figure 2. The general design of a typical microbiological laboratory light microscope. If required, the sample can next be viewed under a higher magnification. Many microscopes hold parfocal objectives that have the same focal positions. Thus only small adjustments will be needed to focus the highe...
image contrast is generated based on scattering and absorbing of the incident light. However, most biological specimens are inherently weakly absorptive and scattering, thus are almost transparent in a conventional bright field microscope. A number of approaches for increasing...
A practical guide for the easy implementation of a Fourier light-field microscope is reported. The Fourier light-field concept applied to microscopy allows the capture in real time of a series of 2D orthographic images of microscopic thick dynamic samples. Such perspective images contain spatial and...
you are equipped for the task at hand regardless of the clearing protocol and imaging solution being used. This light sheet microscope comes in two configurations: it can be fitted with either the Zoom Body Module for easy handling in a multi-user environment, or the Super Plan Module th...
we first conducted numerical simulations in which the required confocal or light-sheet volumes could be replaced by the original synthetic volumes, to quantitatively evaluate their performance in complicated environments (Fig.3and Supplementary Fig.14). To mimic the practical situations for fair compariso...
In this work, we present an actively stabilized, high-resolution, quantitative EUV transmission microscope operating in the silicon transparency window. We demonstrate wavelength-scale resolution combined with material-specific imaging. A critical ingredient is the use of structured illumination to overcome...
angular separation of astronomical objects. To minimize diffraction effects, optical microscopes are sometimes designed to useultraviolet lightrather than longer-wavelength visible light. Nevertheless, diffraction is often thelimiting factorin the ability of amicroscopeto resolve the fine details of objects....
Surface-enhanced Raman spectroscopy (SERS) is a promising analytical method for studying the structure and composition of multi-component media in clinical practice. However, the practical application of SERS imposes several conditions and restrictions depending on the required analytical accuracy and implem...
The optical system of a simple compound microscope consists of four basic elements: a light source, a condenser, an objective lens, and an eyepiece or ocular. The light source in present-day microscopes consists of a tungsten filament light bulb or a higher brightness quartz–halogen bulb. Some...