The intention of this essay is to present a physical illustration of Heisenberg's Uncertainty Principle in the context of transmitted light microscopy. The practical aspects of the example herein are common knowledge to microscopists. The theoretical background using Heisenberg indeterminacy relationships...
When light passes through cells, small phase shifts occur, which are invisible to the human eye. In a phase contrast microscope, these phase shifts are converted into changes in amplitude, which can be observed as differences in image contrast. However, this label-free technique is strongly ...
Optical aberrations limit resolution in biological tissues, and their influence is particularly large for promising techniques such as light-sheet microsco... D Turaga,TE Holy - 《Applied Optics》 被引量: 36发表: 2010年 Minimizing light exposure with the programmable array microscope By incorporating...
The optical lens assembly isplaced in the bottom position of the optical mouse, from Figure 5 can clearlysee that the optical lens component is composed of a light microscope and acircular lens. The mirror will be responsible for the transmission of lightemitted from the light emitting diode li...
Since the first introduction of photoacoustic ophthalmoscopy in 2010 to today, there has been a huge advance in photoacoustic ophthalmoscopy and its application in vivo imaging of the vasculature of the retina in rodents [22]. Recently, the integration of photoacoustic microscopy (PAM) with other im...
The light information in space, wavelength, and time domain at point (1) is shown in Figure 1b. Different spectrum of light is illuminated to the corresponding spatial coordinates of the imaging object. The reflected light returned to the same spatial disperser and was combined into a single ...
microscope. Diffraction effects due to thewavenature of light result in a blurring of the image; the resulting uncertainty in the position of the electron is approximately equal to the wavelength of the light. To reduce this uncertainty, it is necessary to use light of shorter wavelength—e.g...
Typical Application Schematic (Source: Texas Instruments) Fortunately, as part of the FPDLink III family of devices, TI offers devices like the DS90UB962, which is both a 4-channel FPDLink III deserializer and a MIPI-CSI2 hub. This means that it can transmit up to 4 independe...
Abbe, cooperating with Carl Zeiss Inc, summarized his discovery of the microscope imaging principle which states that the... MF Tsai,SJ Chang,CCP Chen,... - 《Proceedings of Spie the International Society for Optical Engineering》 被引量: 8发表: 2009年 A generalized sine condition and ...
The image was recorded at the Bioimaging Facility of the Institute of Science and Technology Austria (IST), using a multiphoton LaVision BioTec TriM Scope microscope. Principle: Just like widefield or confocal fluorescence microscopy, two-photon microscopy is based on fluorophore excitation, which ...