The Use of Western Blot Procedures in the Analysis of Herpes Simplex Virus ProteinsDuring the past few years a multitude of different experimental approaches have been developed to assess the interaction between proteins, of which the use of cross-linking agents and immune electrophoresis are the ...
[Use of western blotting methods in diagnosis of viral infection causing tick-borne encephalitis] 来自 NCBI 喜欢 0 阅读量: 25 作者:A Kowalewska,W Gut,Z Jarzabek,M Kańtoch 摘要: A Western blot method for investigation of antigens and of immunological response to tick-borne encephalitis was ...
A western blot experiment, or western blotting (also called immunoblotting, becausean antibody is used to specifically detect its antigen) was introduced by Towbin, et al. ... The specificity of the antibody-antigen interaction enables a target protein to be identified in the midst of a complex...
The most usual format is the electrophoretic transfer of proteins resolved on a sodium dodecyl sulfate (SDS)-polyacrylamide gel to a membrane, which is known as Western blotting ( 1 ). Polyacrylamide gel electrophoresis (PAGE) has become a standard tool wherever proteins are analyzed. Often, ...
The serodiagnosis of HIV infection is sometimes puzzling due to questionable "indeterminate" results obtained by western blotting (wb), the confirmatory test used world-wide after a positive or borderline ELISA result. These situations cause anxiety in the person being tested and determine additional ...
Researchers pursuing hypotheses about signaling events traditionally start with western blot. In this technique, lysates are pooled and analyzed one protein at a time, and infrequent signaling events may be missed due to insufficient detection sensitivity. Western blotting has limited capability to perfor...
Supernatants enriched in membrane proteins were collected and separated in two equal volumes for subsequent Western blotting experiments in non-reducing and reducing (10% v/v β-mercaptoethanol added to the loading buffer) conditions. Samples were migrated on SDS-PAGE gradient gels (3–8%), dry ...
aWestern-blotting方法检测Bcl-2和Bax的表达结果显示,使用姜黄素治疗的各组与对照组相比,Bcl-2的表达显著增高,Bax含量有所减少。其中,而冰片-姜黄素脂质体组与姜黄素组和姜黄素脂质体组相比,Bcl-2表达有显著增高,Bax的表达有显著降低 The Western-blotting method examines Bcl-2 and the Bax expression result de...
aWestern-blotting方法检测抗凋亡蛋白Bcl-2以及促凋亡蛋白Bax的表达结果显示,使用姜黄素治疗的各组与对照组相比,Bcl-2的表达显著增高,Bax含量有所减少。其中,而冰片-姜黄素脂质体组与姜黄素组和姜黄素脂质体组相比,Bcl-2表达有显著增高,Bax的表达有显著降低 The Western-blotting method examines anti-perishes weakly...
Lysates were sonicated at a force of 4 for 20 strokes (60 Sonic Dismembrator, Fisher Scientific). The lysates were subjected to western blotting analysis. In vitro sgRNA screen in Neuro-2a cell line Neuro-2a cell line with tetracycline-inducible Cas9 expression, (SL508, GeneCopoeia, Rockville,...