Results Characterisation and flow profile of HeLa cells through a constricted microchannel The design of the microchannel is shown in Fig. 2a. The architecture of the fabricated PDMS-based microchannel was visualized under a bright-field microscope (Fig. 2b). The lower panel in Fig. 2b shows a...
Real-time PCR, antibody-based cytokine assays, flow cytometry, and immunofluorescent analyses were performed to determine gene and cellular changes. Results: The mouse model of GFS mimicked the clinical response to mitomycin C. Further analysis of the operated tissues revealed that the post-surgical...
Usefulness of flow cytometry for differential diagnosis of precursor and peripheral T-cell and NK-cell lymphomas: analysis of 490 cases. Pathol Int. 2008;58:89–97. Article PubMed Google Scholar Singh A, Schabath R, Ratei R, Stroux A, Klemke C-D, Nebe T, et al. Peripheral blood ...
—> Blood or Sample test: As we may have already discerned from the dose-escalation study, the phase I trial requires an abundance of blood tests to understand the effect of the drug on the body and how the body gets rid of the drug. All observations and findings are diligently recorded...
currently being used in the research setting have technological limitations, and an important challenge to implementing SCS in the clinic is overcoming errors that may be introduced by amplifying the minute amount of DNA or RNA in a single cell and properly validating the sequencing results. Improved...
The results indicated that AAV9 had reduced efficiency but more penetrating power in crossing barriers through cell-mediated transduction than AAV2. By performing luciferase assay, trans-endothelial assay, permeability assay, flow cytometry, and western blotting, it was found that AAV9 outperformed AAV...
Meanwhile, Baker takes different approaches to study the movement of lipids throughout yeast cells. Drawing on flow cytometry, microscopy and MS imaging techniques, Baker's team analyzes how lipid storage changes with yeast development. "We would like to see metabolomics go beyond just the 'omics...
the investigation development of circulation cytometry in the diagnosis of bovine epidemic conditions ended up being reviewed to be able to offer a reference for future research and application of flow cytometry within the analysis of bovine epidemic diseases.Dengue virus (DENV) could be the cause of...
had on average 35% greater coverage than the rest of the genome (Fig.1cand Supplementary Fig.8), suggesting that these repeats were collapsed in the assembly and may have contributed to the differences in flow cytometry and k-mer genome size estimates. The coding sequence content of SCT is...
Comple- mentary experimental approaches such as quan- titative PCR, flow cytometry, species-specific fluorescence in situ hybridisation or novel combina- tions of single-cell and functional-targeting methods with genomics (Berry et al., 2015) do yield informa- tion on absolute abundances. Yet, ...