or by past experience should guide the experiment. Check this first to ensure the proper stains are being used, and the proper controls are in place to analyze the data.
Next, early apoptosis and viability of the cells was quantified by flow cytometry. Also, the CCK8 assay was performed to analyze the metabolic activity and the cell membrane integrity was determined. Finally, EPO production was detected by Elisa. For in vivo studies, syngeneic C3H mice were ...
If flow cytometry is used to study heterogeneous populations of cells, then it will analyze the subpopulations in a few minutes. Not only is it much faster than other options, the data it produces are also detailed. The analysis includes the percentage of red cells compared with green cells a...
Analyze the fermentation properties of the bacteria in the next element of the flowchart. The bacteria either ferments or oxidizes. Staphylococcus aureus is fermentative as opposed to Micrococcus, which is oxidative. Fermentation analysis is a common step for both Gram-positive and Gram-negative bacte...
We tested the impact of four normalization methods which have either been previously used to analyze the temporal expression organization in cyanobacterial species [11,12,33] (median polishing, quantile normalization) or have become an established standard [34] (cLOESS). Additionally, we tested a ...
Here, we used STORM super-resolution microscopy and single-particle-tracking (SPT) experiments to analyze the differential cell membrane distribution and diffusion behavior of PS, ecto-CRT, and CD47 on adherent HeLa cells. We compared viable cells to cells in early apoptotic stages induced by UVB...
Perspective of Personalized Approaches to Autologous Cell Therapy Taking into account the results of numerous studies discussed, it is important to know the changes of pa- tient's stem/progenitor cells to develop the methods of individual pretreatment of autologous cell material...
Despite these advances, the complexity of the data and the heterogeneity of the tools required to analyze these data can make it difficult for researchers to collaborate effectively to generate the big datasets that AI requires. Efforts such as theFAIRGuiding Principles for scientific dat...
The TCR integrates forces in its triggering process upon interaction with pMHC. Force elicits TCR catch-slip bonds with strong pMHCs but slip-only bonds with weak pMHCs. We develop two models and apply them to analyze 55 datasets, demonstrating the model
Thus, in order to determine the concentration and number of bacteria in the original culture, one needs to make serial dilutions where one dilution is used to make the next. Then, one needs to plate known volumes of some of the more dilute solutions onto agar plates co...