The most critical requirement for efficient and effective flow cytometry analysis is that the sample be a single-cell suspension. This helps ensure that every cell is analyzed independently. Topics in this section...
or by past experience should guide the experiment. Check this first to ensure the proper stains are being used, and the proper controls are in place to analyze the data.
when relying on flow cytometry phenotyping alone to define Tregs, inflammatory T cells could be a wolf in sheep’s (Treg’s) clothing and lead to incorrect data interpretation.
Flow CytometryBlotting, WesternSignal TransductionBACKGROUND: Heterogeneously distributed hypoxic areas are a characteristic property of locally advanced breast cancers (BCa) and generally associated with therapeutic resistance, metastases, and poor patient survival. About 50% of locally advanced BCa, where ...
The TCR integrates forces in its triggering process upon interaction with pMHC. Force elicits TCR catch-slip bonds with strong pMHCs but slip-only bonds with weak pMHCs. We develop two models and apply them to analyze 55 datasets, demonstrating the model
Complexities of Big Data Despite these advances, the complexity of the data and the heterogeneity of the tools required to analyze these data can make it difficult for researchers to collaborate effectively to generate the big datasets that AI requires. Efforts such as the FAIR Guiding ...
They used their platform to analyze the transcriptome of almost 50,000 mouse retinal cells and were able to identify several transcriptionally different populations. One big advance in the field of single immune cell analysis was the sequencing of both immune receptor chains from millions of ...
: Worth Two Billion?” His own expectations of AI are more measured: “AI is going to be very good at digging through what we've already found,” but added “We just don’t know enough about cells, about organisms, and about disease” to feed the AI an adequate data set to analyze...
[133]. Preliminary data in DLBCL, HL and FL suggest that ctDNA has promise as a marker of MRD and predictor of recurrence [134,135,136,137]. In an ongoing study, we aim to prospectively analyze ctDNA levels in patients with newly diagnosed FL to benchmark levels in correlation to ...
The time- and effort-saving benefits of flow cytometry are crystal clear. Flow cytometry instruments can sort and analyze thousands of cells per minute, and theFlow Cytometry Panel Buildersimplifies this process. By facilitating fast and optimum panel design, the panel builder frees up your time....