If you're new to flow cytometry, and/or use one of the modern benchtop flow cytometers which don't require, or even allow, you to mess with the guts of the instrument, you may not care much about how flow cytometers work. A lot of highly competent molecular biologists, immunologists,...
After completing the perfect staining and cytometry run, the hard work begins – data analysis. To properly identify the cells of interest, it is critical to pull together knowledge of the biology with the controls run in the experiment to properly place the regions of interest that will be di...
T cells that express CD45RO/CD44 (human/mouse) and do exert effector cytokines can be defined as memory cells. To distinguish between an activated and resting memory state, a key difference is their polyfunctionalityex vivo, with true resting memory cells typically able to secrete a panoply of...
We picked a handful of Santa Cruz’s most cited antibodies and performed head-to-head comparisons on multiple applications ranging from western blot to flow cytometry. Our decision to test Santa Cruz’s most cited antibodies was risky, but deliberate. We understood that their top selling ...
Light scatter is unfortunately one of the more misunderstood concepts in cytometry. When you firstlearn flow cytometry, you were likely told the misleading phrase, “Forward scatter signal intensity is proportional to cell size, and side scatter signal intensity is proportional to cell granularity...
Not sure whether to do it yourself or outsource the flow cytometry procedures? If you are designing your own flow lab, we can identify flow cytometers and associated instruments that are best suited for your purposes. On the other hand, if you need help identifying, negotiating with, or audi...
To determine the number of cells actively dividing within a culture, cell proliferation assays typically measure DNA synthesis or overall DNA content within replicating cells through imagine, flow cytometry, immunofluorescence, or enzyme-linked immunosorbent assays (ELISA). ...
activated T cells often up-regulate CD25, and FOXP3 expression has been found on ‘effector’ (non-suppressive) T cell lineages.Therefore, when relying on flow cytometry phenotyping alone to define Tregs, inflammatory T cells could be a wolf in sheep’s (Treg’s) clothing and lead to incorr...
aFifty-four CHB patients and 31 liver cirrhosis (LC) patients were consecutively collected, as well as 11 healthy subjects as controls. Expression levels of IFNAR1 and IFNAR2 in peripheral blood lymphocytes and monocytes were measured by flow cytometry. IFNAR1 and IFNAR2c mRNA were detected by...
bioAffinity Technologies hired ScienceSoft to help in the development of its automated data analysis software for detection of lung cancer using flow cytometry. Our project required a large amount of industry specific methodology and algorithms to be implemented into our new software connected to EHR/...