After completing the perfect staining and cytometry run, the hard work begins – data analysis. To properly identify the cells of interest, it is critical to pull together knowledge of the biology with the controls run in the experiment to properly place the regions of interest that will be di...
If you're new to flow cytometry, and/or use one of the modern benchtop flow cytometers which don't require, or even allow, you to mess with the guts of the instrument, you may not care much about how flow cytometers work. A lot of highly competent molecular biologists, immunologists,...
T cells that express CD45RO/CD44 (human/mouse) and do exert effector cytokines can be defined as memory cells. To distinguish between an activated and resting memory state, a key difference is their polyfunctionalityex vivo, with true resting memory cells typically able to secrete a panoply of...
Not sure whether to do it yourself or outsource the flow cytometry procedures? If you are designing your own flow lab, we can identify flow cytometers and associated instruments that are best suited for your purposes. On the other hand, if you need help identifying, negotiating with, or audi...
activated T cells often up-regulate CD25, and FOXP3 expression has been found on ‘effector’ (non-suppressive) T cell lineages.Therefore, when relying on flow cytometry phenotyping alone to define Tregs, inflammatory T cells could be a wolf in sheep’s (Treg’s) clothing and lead to incorr...
skin bacteria, Staphylococcus aureus, offers an excellent place to start, with each step in the flowchart set in a geometric shape such as a rectangle, oval, square or box with lines connecting each subsequent step beneath for portrait-aligned flowcharts and horizontally for landscape oriented ...
we have begun putting our own antibodies to the test. We picked a handful of Santa Cruz’s most cited antibodies and performed head-to-head comparisons on multiple applications ranging from western blot to flow cytometry. Our decision to test Santa Cruz’s most cited antibodies was risky, but...
Using flow cytometry to look at uniform cells populations has the benefit of always highlighting any non-uniformity. It also takes off any debris or dead cells when providing the final data. This level of accuracy beats that of the competition. ...
To detect leukemic cells at a sensitivity threshold of less than 0.01%, at minimum a 6-color flow cytometry needs to be performed.2 “If the level of sensitivity is 0.1% or worse, it’s going to be very hard to be able to utilize the data for MRD-directed therapy because you’re ...
Cells are the basic units of life and contain cytoplasm, DNA, ribosomes and a cell membrane. When using a light microscope to examine living cells, you may want to calculate the cell density. A variety of cell counting methods exist for exactly this purp