Induction of ICOS expression was analyzed on CD8–CD3+ lymphocytes using three-color flow cytometry. Blood from a patient with diagnosed ICOS deficiency was also analyzed. Results: Whole-blood stimulation with PMA and ionomycin for 20 h resulted in a significant induction of ICOS expression on CD8...
sensitive and specific method with results that correlate well with those of RQ-PCR for the fusion transcript. In addition, usage of flow cytometry gives the advantage of simultaneously investigation of surface markers commonly applied in the clinical...
blood group Ab in sera of rhesus or cynomolgus monkeys was detected by flow cytometry after adding secondary specific fluorescence-labeled anti-human IgG or IgM Ab.Human healthy blood donor sera were used as controls.The results revealed that,using clear monkey sera,which were pre-absorbed on ...
Flow cytometry can be used to help researchers perform high-throughput and quantitative analysis of the tumor’s “microenvironment”, peripheral blood and the immune system in other host tissues, to more clearly predicting the efficacy of immunotherapy and to help the large-scale immunotherapy methods...
摘要: Jensen JE, Jensen PB, Nielsen B, Kemp E.关键词: linfocitos, lymphocytes, rat, tail, rata, cola (anatomia animal), heart, prelevement sanguin, lymphocyte, blood sampling, coeur, corazon, rats, queue, muestreo sanguineo DOI: 10.1111/j.1365-2885.1996.tb00073.x 被引量: 5 ...
Using the power ofspectral flow cytometryand cell sorting, researchers can now identify and sort cells based on new combinations of markers. However, the increased complexity of panel design and complex subset characterization req...
Flow cytometry using annexin V can detect early apoptosis in peripheral blood stem cell harvests from patients with leukaemia and lymphoma. Parker AC (1998) Flow cytometry using Annexin V can detect early apoptosis in peripheral blood stem cell harvests from patients with leukaemia and lymphoma... ...
6 While FVIII is widely measured intracellularly with immunohistochemistry staining methods, a reliable protocol for flow cytometry (FC) staining is still not available. Few publications are describing controversial results on FC detection of FVIII in blood cells,7,8 with the clearest results only ...
Aged whole human blood (AllCells) lysed with ammonium chloride lysis buffer. Image processing was done using the Cells Model_Speed Optimized model. Statistics shown are % gated.Altering Gating and Analysis through Integration of High Speed, High Resolution Imaging wit...
Whole Blood Activation Results in Enhanced Detection of T Cell and Monocyte Cytokine Production by Flow Cytometry An excellent monitor of the immune balance of peripheral circulating cells is to determine their cytokine production patterns in response to stimuli. Using... CF Sams,BE Crucian 被引量:...