FLOW CYTOMETRY WHOLE BLOOD SAMPLESBlood, R E D
Here we describe a novel flow cytometry (FLOW)-based assay to identify and quantify NETs using antibodies against key NETs constituents, specifically DNA, modified histones, and granular enzymes. This method is applicable to both murine and human samples for the assessment of induced NETs in vitro...
The ability of flow cytometry to resolve multiple parameters was used in a microsphere-based flow cytometric assay for the simultaneous determination of several cytokines in a sample. The flow cytometer microsphere-based assay (FMBA) for cytokines consists of reagents and dedicated software, specifically...
In patient samples, this has the advantage of only requiring a small amount of blood for flow cytometry analysis. This can be applied to the analysis of cytokines such as Interferon (IFN gamma). Fig.8: C57BL/6 mouse splenocytes were stimulated with PMA and Ionomycin (4h) in the ...
Flow cytometry analysis of pan-T-, CD4(+)-, CD8(+)-, and pan-B lymphocytes in blood samples obtained from healthy, nontreated rats: comparison of lymphocyt... Flow cytometry analysis of pan-T-, CD4(+)-, CD8(+)-, and pan-B lymphocytes in blood samples obtained from healthy, non...
Detection of intracellular factor VIII protein in peripheral blood mononuclear cells by flow cytometry BioMed Res. Int., 2013 (2013), p. 793502 Google Scholar 8 A. Ishaque, J. Thrift, J.E. Murphy, K. Konstantinov Cell surface staining of recombinant factor VIII is reduced in apoptosis resista...
This protocol describes the immunophenotyping of cells in a whole blood sample by flow cytometry with minimal sample manipulation, thereby preserving cell structure and function while also reducing cell loss [1,2]. Whole blood samples st...
flow cytometry with specific and sensitive detection of the BCR-ABL1 positive leukemic cells using thein situPLA. The assay provides rapid detection of BCR-ABL1 using flow cytometry, particularly attractive in the diagnostic setting, and it represents a novel tool for identification and enumeration ...
Focuses on the use of flow cytometry methods for counting of CD34 cells in blood. Use of single- or dual-laser excitation in the methods; Analysis of the cells in unlysed whole blood, stained with monoclonal antibodies; Identification of CD34 cells with class III monoclonal antibodies by ...
Reagent and method of use thereof for the automatic determination in flow cytometry at least one leukocyte sub-population from whole blood. The invention relates to a reagent and method of use thereof AUTOMATIC FOR DETERMINING AT LEAST ONE subpopulation LEUKOCYTE A whole blood sample. This reagent ...