An E3 ligase TRAF7 regulates time-of-the-day-dependent expression of DBP proteins via the ubiquitin-proteasome system, providing insight into the posttranslational regulation of clock proteins in the mammalian circadian clockwork. Shusaku Masuda ...
Ubiquitin and its associated enzymes act as cellular guardians to ensure precise spatio-temporal control of key molecules during each of these important cellular processes. Loss of precision results in numerous examples of embryological disorders or even cancer. This Review discusses the crucial roles ...
Ubiquitin ligase enzymes promote substrate protein ubiquitination, a post-translational modification whereby the 76-amino acid protein ubiquitin is covalently bound to substrate proteins. Ubiquitination may target substrates for proteasomal degradation or regulate substrate function in a degradation-independent ma...
2016). There are about 40 different E2 enzymes in humans, which share a conserved core domain and perform a wide range of functions in the cell. However, in general, E2s are mostly responsible for carrying the ubiquitin molecule to the next enzyme, the E3 ligase (Stewart et al., 2016)....
Ubiquitin and a lysine residue in the target protein. This process of conjugation, referred to as ubiquitination or ubiquitylation, is a multistep process that requires three enzymes: a Ubiquitin activating(E1) enzyme, a Ubiquitin conjugating (E2) enzyme, and a Ubiquitin ligase (E3). Ubiquitinatio...
E3 ubiquitin (Ub)-ligase enzymes, which are classified into different families depending on their structural and functional features, confer the specificity of substrate and are the best characterized components of the ubiquitination cascade. E3 Ub-ligases of different families have been shown to be ...
英文别名:Ubiquitin-Conjugating Enzyme E2 H, UbcH2, Ubiquitin Carrier Protein H, Ubiquitin-Conjugating Enzyme E2-20K, Ubiquitin-Protein Ligase H, UBE2H 描述:Recombinant Human Ubiquitin-Conjugating Enzyme E2 H is produced by our E.coli expression system and the target gene encoding Met1-Leu183 is...
These two related, multi-subunit E3 ubiquitin ligase enzymes, APC and SCF are thought to be the major driving forces governing proper cell cycle progression. Defective cell cycle regulation leads to genomic instability and ultimately, cancer development. Selective degradation of key cell cycle ...
enzymes,UBC12orUBE2F.TheseenzymesdockwithanE3ligasecomplex,which providesadditionalsubstratespecificity.UBC12neddylatesCUL1through4,whileUBE2F neddylatesCUL5[14]. Here,wetesttheimpactofneddylationonHIVinfectionandonHIVfunctionsmediated throughculltubiquitinligases.Inthisworkweinhibitedneddylationusingeither ...
W. Ubiquitin-like protein activation by E1 enzymes: the apex for downstream signalling pathways. Nature Rev. Mol. Cell Biol. 10, 319–331 (2009). CAS Google Scholar Berndsen, C. E. & Wolberger, C. New insights into ubiquitin E3 ligase mechanism. Nature Struct. Mol. Biol. 21, 301–...