serial in vitro passage, suggested that passage-induced changes may lead to notable changes of biological characteristics and several molecular transitions in cancer cell lines, indicating the necessity to shorten experiment-span and accomplish experiments with the same or similar passage cancer cell ...
The extent of resection is affected by tumor size, location and patient characteristics (ex. age and patient performance status) (Stummer et al., 2006, Westphal et al., 2003, Stupp et al., 2005). Glioblastoma multiforme is characterized with high proliferative activity (Schröder et al., ...
800 × g; duration, 20 min) with lymphocyte separation medium (density, 1.077±0.002). The mononuclear cells were re-suspended in cell culture medium (Beyotime Institute of Biotechnology, Shanghai, China) to a density of 4.0×106/ml. On day 0, the cells...
4)Cell line U87细胞系U87 5)glioma cellU87胶质瘤 1.Knockdown of OPN by OPN-RNAi inhibits growth and invasiveness of U87 glioma cellsOPN基因RNAi对U87胶质瘤细胞生长和侵袭的抑制作用 6)U87 cellsU87细胞 1.Effects of VEGFR-2 siRNA on biological characteristics of VEGFR-2 receptor-positive U87 cells...
Thymoquinone is one of the drugs that exhibits inhibitory characteristics based on in vivo and in vitro models. Aim: This study further investigates the effects of thymoquinone on human global gene expression from opioid receptor expressing cell line (OREC) using RNA sequencing technology. The cell...
MATERIALS AND METHODS: U87MG cells (Human glioblastoma cell line) were treated with Rg3, measured cell viability using MTT assay and trypan blue exclusion assay. The apoptotic characteristics were detected by TUNEL assay, flow cytometry analysis and western blotting. The molecular mechanisms wer...