This confirms previous results that the loss of PD-L1 is important for regulating immune functions. This is the first time CRISPR/Cas9 technol- ogy has been used with dual-sgRNAs and a repair template to target PD-L1 in human glioblastoma cells. Dual- sgRNA CRISPR/Cas9 + HDR to ...
This confirms previous results that the loss of PD-L1 is important for regulating immune functions. This is the first time CRISPR/Cas9 technology has been used with dual-sgRNAs and a repair template to target PD-L1 in human glioblastoma cells. Dual-sgRNA CRISPR/Cas9 + HDR to knockout ...
For cell cycle analysis, cells were treatment with CIK for 72 h and the tumor cells were collected and incubated with PI away from light for 30 min. The cell cycle was determined by flow cytometry and calculated using ModFit3.0 software (Verity Software House, Topsham, ME, USA). Western b...
[29]. Interestingly, this peptide contains a sequence WR, predicting the same structural characteristics of the W2K3sequence in the AaTs-1 peptide. Therefore, these two dipeptides were aligned together in the guiding local alignment. The conformations of I1and S4of AaTs-1 were only modeled ...
Standardized orthotopic xenografts in zebrafish reveal glioma cell-line-specific characteristics and tumor cell heterogeneity. Dis. Model. Mech. 2016, 9, 199–210. [CrossRef] [PubMed] 12. Geiger, G.A.; Fu, W.; Kao, G.D. Temozolomide-mediated radiosensitization of human glioma cells in a ...