Gibco cell culture media & reagents Gibco growth factors Gibco FBS Countess II FL Automated Cell Counter MODIFY Lipofectamine transfection reagents Neon electroporation transfection system Invitrogen RNAi products Recombinant proteins Dynabeads cell separation technology ...
(The U87IE1 cell line is a generous gift from Charles Cobbs.) METHODS: Approximately 5,000 U87IE1 and U87MG cells in normal culture media were placed into wells of a 96-well plate. After 24 hours, the media was replaced with culture media containing temozolomide in increasing concentration...
In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is ...
Therefore the present in vitro study aimed at investigating the contribution of the committed step of de novo SL biosynthesis, individual members of the S1P receptor family, and the putative S1P efflux pump ABCA1 to GBM cell proliferation. 2. Materials and methods 2.1. Materials Cell culture ...
In addition to intracellular and EV-derived Hsps, it was also demonstrated that at least Hsp70 and Hsp60 were shed from glioma cells into the cell culture media (supernatant, SN). In the extracellular milieu, Hsp70 level was elevated in a toxic dose of TMZ (750 μM), which might explain...
Gibco cell culture media & reagents Gibco growth factors Gibco FBS Countess II FL Automated Cell Counter MODIFY Lipofectamine transfection reagents Neon electroporation transfection system Invitrogen RNAi products Recombinant proteins Dynabeads cell separation technology DETECT Invitrogen imaging reagents Invitrogen...
Gibco cell culture media & reagents Gibco growth factors Gibco FBS Countess II FL Automated Cell Counter MODIFY Lipofectamine transfection reagents Neon electroporation transfection system Invitrogen RNAi products Recombinant proteins Dynabeads cell separation technology DETECT Invitrogen imaging reagents Invitrogen...