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(The U87IE1 cell line is a generous gift from Charles Cobbs.) METHODS: Approximately 5,000 U87IE1 and U87MG cells in normal culture media were placed into wells of a 96-well plate. After 24 hours, the media was replaced with culture media containing temozolomide in increasing concentration...
In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is ...
The cell apoptosis index was measured using the DeadEnd Fluorometric TUNEL System (Promega) following the manufacturer's instructions. Caspase activity assay. Cells treated with HAMLET, OA or HLA alone for 3 h were harvested and incubated in culture media with 10 mmol/l of FAM-LETD- FMK (...
In addition to intracellular and EV-derived Hsps, it was also demonstrated that at least Hsp70 and Hsp60 were shed from glioma cells into the cell culture media (supernatant, SN). In the extracellular milieu, Hsp70 level was elevated in a toxic dose of TMZ (750 μM), which might explain...
cell dye. Briefly, U87MG and U373MG cells were seeded in a 6 well plate at 2 × 105cells/well, incubated for 24 h and treated with SFN [20–80 µM] for 48 h at 37 °C in normoxic or hypoxic conditions. At the end of the treatment, the culture medium was discarded, and 50 ...