(The U87IE1 cell line is a generous gift from Charles Cobbs.) METHODS: Approximately 5,000 U87IE1 and U87MG cells in normal culture media were placed into wells of a 96-well plate. After 24 hours, the media was replaced with culture media containing temozolomide in increasing concentration...
(b) EREG protein levels in cell-conditioned media as determined by ELISA. Results are mean values ± SD. The dotted line represents the limit of detection of the measure. (c) The chicken egg model. Cells were deposited onto the chicken CAM and tumors were allowed to grow for 4 days. ...
we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost...
Cell culture and transfection U87 cells (ATCC, Manassas, VA) and MDA MB 231 cells (gift from Gadad Lab) were cultured in growth media consisting of Dulbecco’s-Modified Eagle Medium (DMEM, Sigma-Aldrich, St Louis, MO) supplemented with 10% fetal bovine serum (FBS; Sigma-Aldrich, St Loui...
Human U87 Astrocytoma Cell Invasion Induced by Interaction of βig-h3 with Integrin α5β1 Involves Calpain-2 英文参考文献.doc,HumanU87AstrocytomaCellInvasionInducedby Interactionofbig-h3withIntegrina5b1InvolvesCalpain- 2 JieMa1.,WeiCui2.,Shi-mingHe1,Yon
C) Western blotting analysis of Hsp70 and Hsp60 expression in cell lysate (CL), extracellular vesicles (EVs), and supernatant-cell media (SN). While using GAPDH as a loading control for CL, Ponceau staining was performed for SN and EVs. p-Values were determined using one-way ANOVA ...
Our results showed that ATO modified the expression of a significant number of cancer-related miRNAs in glioblastoma cell line; most of these especially the upregulated miRNAs, were known as tumor suppressors with extensive roles in cancer associated processesand pathways. This study supports the ...
Our results showed that ATO modified the expression of a significant number of cancer-related miRNAs in glioblastoma cell line; most of these especially the upregulated miRNAs, were known as tumor suppressors with extensive roles in cancer associated processes and pathways. This study supports the ...
The control cells were treated with an equivalent volume of vehicle in growth media. Total RNA was isolated from the U87 MG cells using the RNA mini kit's protocol (Geneaid) and quantified using a BioDrop 2000c spectrophotometer. After mRNA enrichment and cDNA synthesis, the amplified cDNA ...
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