The whole process can be divided in two: the molecular biology of gene isolation and expression, and the purification from the recombinant cells. But the latter can be strongly influenced by the former; indeed, the form in which the protein is expressed can include the means of purification, ...
Characterization of a cDNA encoding the 70-kDa single-stranded DNA-binding subunit of human replication protein A and the role of the protein in DNA replication J. Biol. Chem. (1991) M Ishiai et al. Purification, gene cloning and reconstitution of theheterotrimeric single-stranded DNA-binding ...
The present study describes an efficient method for isolation and purification of protein extracts from four types of human teeth i.e. molar, premolar, canine, and incisor. Detailed structural characterization of these protein extracts was done by Fourier transform infrared spectroscopy (FTIR) and ...
Protein interactions mediate a wide spectrum of functions in various cellular contexts. Functional versatility of protein complexes is due to a broad range of structural adaptations that determine their binding affinity, the number of interaction sites, and the lifetime. In terms of stability it has...
Several polyclonal antisera specific for each of the protein kinase C isotypes α, β1, β2 and γ have been generated and used to monitor the purification and subsequenct separation of these polypeptides. A simple protocol has been developed for the efficient co-purification of these isotypes...
that enables the purification of cell types purely based on the native characteristics of the cells, such as shape, size and granularity, which can be measured by a flow cytometer. This new approach makes it possible to purify a cell type of choice without having to resort to genetically modi...
of sodium hyaluronate shall provide different dossiers when apply for CFDA registration, for example, the animal source HA filler requires for sufficient study of its raw materials suppliers, supplied goods, inactivated virus, immune prototype, protein purification process, etc; HA filler prepared from...
Purification of a novel class of coated vesicles mediating biosynthetic protein transport through the Golgi stack. We describe a scheme for the purification of the non-clathrin-coated vesicles that mediate transport of proteins between Golgi cisternae and probably from ... V Malhotra,T Serafini,L ...
Infant formulas are produced from oil-in-water emulsions, which are mainly stabilized by proteins, usually of dairy origin, and other common nonprotein emulsifiers (such as lecithin) (McCarthy et al., 2015; McSweeney et al., 2008). Proteins are surface active polymers that contain residues ...
The mutatedndhD genes in the transformants were segregated to homogeneity (by successive streak purification) as determined by polymerase chain reaction amplification. Determination of Growth Characteristics Wild-type and mutant strains grown under 3% CO2 were collected and resuspended in fresh BG11 ...