Methods EnzymolMethods in EnzymologyParker PJ, Marais RM. Purification of protein kinase C isotypes from bovine brain. Methods Enzymol. 1991; 200 :234–241.Parker, P. J., and R. M. Marais. 1991. Purification of protein kinase C isotypes from bovine brain, p. 234-241. In B. M. S...
1a). Specifically, these mice constitutively express CycB1-GFP, a fusion protein of the N-terminal portion of cyclin B1 and eGFP that behaves like full-length cyclin B1 in terms of expression during the cell cycle, i.e. it is degraded in G0/G1 phases so that cells are GFP negative (G...
Protein interactions mediate a wide spectrum of functions in various cellular contexts. Functional versatility of protein complexes is due to a broad range of structural adaptations that determine their binding affinity, the number of interaction sites, and the lifetime. In terms of stability it has...
2.2. Methods 2.2.1. Emulsion preparation The water-soluble components were dispersed in distilled water under magnetic stirring at room temperature overnight, ensuring complete dissolution of the protein. The pH of the dispersions was adjusted to 6.8 using KOH (10% wt. %). Emulsions were prepared...
Based on the intrinsic and catalyzed off-rates, the DM susceptibility8 can be calculated (see Methods) as a measure of specific peptide exchange rate enhancement. Our data show that DM clearly enhances peptide exchange for all of the investigated allotypes, but to a different extent (Fig. 2b...
Several polyclonal antisera specific for each of the protein kinase C isotypes α, β1, β2 and γ have been generated and used to monitor the purification and subsequenct separation of these polypeptides. A simple protocol has been developed for the efficient co-purification of these isotypes...
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Improved methods for purification and depletion of monocytes from bovine peripheral blood mononuclear cells. Functional evaluation of monocytes in response... We have compared different techniques for the enrichment and depletion of monocytes from bovine peripheral blood mononuclear cells. Adherence to plasma...
kinetics and persistence of circulating antibodies recognizing different portions of the mRNA-coded proteins, we performed an isotype-specific plasma screen28 against the following SARS-CoV-2 targets: S1 and S2 subunit domains of the spike protein, RBD of the S1 subunit, and the N-terminal domain...
. Using the GET model, which had seen no lentiMPRA data, we inferred the activity of the mini promoter in the corresponding chromatin context and averaged over all insertions to obtain a mean readout indicative of the regulatory activity (Fig.2aandMethods: ‘LentiMPRA zero-shot prediction’)....