Protein purification techniques (2nd ed.), Roe, S. (ed.); and protein purification applications (2nd ed.), Roe, S. (ed.)doi:10.1002/bmb.2002.494030019999JamesClemsonZimmermanClemsonWileyBiochemistry & Molecular Biology Education
6 ATP synthase is a highly complex molecular machine 7 无 片 无 片 8 9 Basic pr iple of protein purification 10 protein purification techniques ➢Based on physical and chemical characteristics: size dialysis, gel filtration chromatography, charge ion exchange chromatography hydrophobicity hydrophobic ...
Purification of rat liver glucokinase. A new purification method for rat liver glucokinase was developed. Glucokinase was purified to homogeneity in a yield of 70% in 5 days. The procedure consi... Y Toyoda,I Miwa,M Kamiya,... - 《Preparative Biochemistry》 被引量: 7发表: 1994年 加载更多...
Site-specific modification of interleukin-2 by the combined use of genetic engineering techniques and transglutaminase. Biochemistry 35, 13072–13080 (1996). CAS PubMed Google Scholar Fernández-Suárez, M. et al. Redirecting lipoic acid ligase for cell surface protein labeling with small-molecule ...
proteinpurificationusingaffinity chromatographytechniques (Bell et al., 2013; Terpe, 2003). The smallest peptide tags consist of less than 10amino acids, e.g., the polyarginine-tag, polyhistidine-tag, FLAG octapeptide and Strep-tag. Fusing these tags at the N- or C-terminus of the target ...
Guide to Protein Purification译者: Burgess, Richard R;Deutscher, Murray P. 出版商: Elsevier Science 出版年: 2009 ISBN: 9780080923178 分类: [Q51 蛋白质] 语种: ENG 简介 Guide to Protein Purification, Second Edition provides a complete update to existing methods in the field, reflecting the...
To decrease the UbiX sample contamination by unbound FMN during washing with 70% acetonitrile, FMN (1 mM) can be added at the cell lysis step during protein purification. In this case, FMN addition in step 2 is no longer needed as the UbiX protein (AF1214) will be mostly saturated with...
This second edition of "Membrane Protein Purification and Crystallization, A Practical Guide" is written for bench scientists working in the fields of biochemistry, biology, and proteomic research. This guide presents isolation and crystallization techniques in a concise form, emphasizing the critical asp...
Protein concentration quantitation is an integral part of any laboratory workflow involving protein extraction, purification, labeling or analysis. Pierce Protein Assays provide a wide range of options for accurate protein concentration determin...
2. Identification, Production, and Purification of Protein-Based Nanoparticles The identification of PNPs has become easier due to the accessibility of genomic data and the development of powerful bioinformatics software for genome mining. For example, Giessen and Silver used this combined approach to ...