Protein purification techniques (2nd ed.), Roe, S. (ed.); and protein purification applications (2nd ed.), Roe, S. (ed.)doi:10.1002/bmb.2002.494030019999JamesClemsonZimmermanClemsonWileyBiochemistry & Molecular Biology Education
A new purification method for rat liver glucokinase was developed. Glucokinase was purified to homogeneity in a yield of 70% in 5 days. The procedure consi... Y Toyoda,I Miwa,M Kamiya,... - 《Preparative Biochemistry》 被引量: 7发表: 1994年 Protein purification techniques Second Edition A...
Site-specific modification of interleukin-2 by the combined use of genetic engineering techniques and transglutaminase. Biochemistry 35, 13072–13080 (1996). CAS PubMed Google Scholar Fernández-Suárez, M. et al. Redirecting lipoic acid ligase for cell surface protein labeling with small-molecule ...
Higher organisms such as insects, plants, and mammalian cells, and even live animals, have been used for expression of recombinant proteins; however, transfection, cell/organism culture, and protein expression and purification tend to be more difficult in these systems than in bacteria. After ...
2. Identification, Production, and Purification of Protein-Based Nanoparticles The identification of PNPs has become easier due to the accessibility of genomic data and the development of powerful bioinformatics software for genome mining. For example, Giessen and Silver used this combined approach to ...
The earliest application can be traced back to the use of peptide/protein tags to enable one-step protein purification using affinity chromatography techniques (Bell et al., 2013; Terpe, 2003). The smallest peptide tags consist of less than 10 amino acids, e.g., the polyarginine-tag, poly...
Guide to Protein Purification译者: Burgess, Richard R;Deutscher, Murray P. 出版商: Elsevier Science 出版年: 2009 ISBN: 9780080923178 分类: [Q51 蛋白质] 语种: ENG 简介 Guide to Protein Purification, Second Edition provides a complete update to existing methods in the field, reflecting the...
371 Date Apr. 3, 1996 Sec. 102(e) Date Apr. 3, 1996 PCT Filed Jul. 6, 1994 PCT Pub. No. WO95/01797 PCT Pub. Date Jan. 19, 1995This invention relates to the application of immobilized metal affinity chromatography to the purification of complement receptor proteins....
Optimizing CF reactions in order to decrease protein aggregation during the purification processes and increasing the quality of the protein purification, especially when using the CECF method, is strongly required. Another point to address in the field of CFPS is to decrease the costs of production...
This second edition of "Membrane Protein Purification and Crystallization, A Practical Guide" is written for bench scientists working in the fields of biochemistry, biology, and proteomic research. This guide presents isolation and crystallization techniques in a concise form, emphasizing the critical asp...