In contrast to conventional PEG/dextran two-phase systems, quantitative protein recovery from liquid phases containing polyampholytes could be achieved simply by iso-electric precipitation of the polymer.doi:10.1016/0141-0229(88)90007-5Peter Hughes...
CPC systems have been optimized to achieve high stationary phase retentions for ATPSs and optimized mixing between stationary and mobile phases [36]. CPC case studies with ATPSs have been performed in the past for high-resolution separations of model proteins [37], and for the purification of ...
Size-exclusion chromatography applied to the purification of whey proteins from the polymeric and saline phases of aqueous two-phase systems Size-exclusion chromatography (SEC) was applied to purify cheese whey proteins 伪-lactalbumin and 尾-lactoglobulin which were present, respectively, in the... EE...
Faust's life experience of these two phases of the ideals and social ideals, Faust has a different in these two life stages of the pursuit of experience refining summed up the spirit of "Faust" Faustian spirit of the many connotations of self-improvement, goodwill, self-transcendence. ...
In a bio-separation process, the A. oryzae alpha-amylase could be isolated with ATPSs PEG 2000/Pi or PEG 8000/Pi with a high recovery, in the top or bottom phases, respectively. 展开 关键词: Alpha-amylase Partition Aqueous two phase systems Protein purification ...
The fusionated proteins to said polypeptides with choline affinity are preferably located in one of the phases, while most of the cell extract proteins tend to go to the opposite phase. After a series of washings for removing the rest of the not desired material, this location can be ...
Partitioning in aqueous two-phase system (ATPS) provides a rapid and gentle means of separation of soluble as well as particulate biomaterials, e.g. proteins, nucleic acids, cells, viruses, organelles, and membranes. Partitioning between the two phases is a complex phenomenon, guided mainly by ...
The pathway consists of two distinct phases, an oxidative phase that allows reduction of NADP+ to NADPH, and a non-oxidative phase in which R5P is reversibly converted to glycolytic intermediates such as glyceraldehyde-3-phosphate (GAP) and fructose-6-phosphate (F6P). The pentose phosphate ...
Hence, there has been continuous interest in developing new polymers having properties that can enhance partitioning of substances between the phases. Unlike the PEG–dextran system, where the phase-forming components are neutral, some of the new types of aqueous two-phase systems being developed ...
Chemical potential is used to describe the phase equilibria between the two aqueous phases and the thermodynamic parameters required are obtained from experimental data. Empirical correlations are used to represent the more complex behaviour of protein partitioning, as currently no thermodynamic prediction ...