Real-time PCR, also known asquantitative PCR or qPCR, is a technique for monitoring and quantifying PCR results in real-time by labeling DNA molecules with fluorescent dye. Reverse-Transcriptase(RT-PCR) converts RNA to DNA in the process of producing complementary DNA (cDNA). Nested PCRlowers ...
The results of PCR–RFLP completely coincided with those of sequence analysis, and thus PCR–RFLP is a useful technique for determining the ITS1 type in Fasciola species. 展开 关键词: characterization DNA infections molecular genetics nucleotide sequences parasites parasitology parasitoses polymorphism ...
A consensus multiplex PCR (CM-PCR) technique was developed to detect high-risk (HPV 16/18), low-risk (HPV 6/11), and over 40 other types of human papillomavirus (HPV), separately but simultaneously, by mixing three pairs of consensus primers in the same PCR mixture, for gene ...
The cDNAs are amplified by using PCR. Then these immobilized on a solid support made up of nylon filtre of glass slide (1 x 3 inches). The probe DNA are loaded into a a spotting spin by capillary action. Small volume of this DNA preparation is spotted on solid surface making physical c...
Microfludic technology offers a compelete new solutions to achieve a small size for a fluid system since lots of tubes are replaced by fluidic path in-between plactic plates. With further exploring the technique, scientists and senior technicians are investigaing the method of integrating the ...
Moreover, single DRG neurons responding to cutaneous stimuli are recorded using an in vivo whole-cell patch clamp technique and classified by neuron-type genetic markers. Small diameter DRG neurons are classified into one type of low-threshold mechanoreceptor and five types of mechanoheat nociceptors...
(WGS) is considered as an advanced genotyping approach with the highly discriminative tool for strain typing inE. amylovora12. However, this technique is time consuming and costly. In particular, regarding reproducibility, the reference genome and the analytical pipeline and settings should be ...
A Microplate Method for Isolation of Viruses from Infants and Children with Acute Respiratory Infections Between December 1984 and December 1986, a microplate technique was adopted for isolation of viruses from infants and children with acute respiratory infec... Y Numazaki,T Oshima,A Ohmi,... -...
The emergence of a mature nervous system requires a significant refinement of the synaptic connections initially formed during development. Redundant synaptic connections are removed in a process known as synapse elimination. Synapse elimination has been
Considering the scarcity of research on the antitumor immune effects of LUAD pertaining to T cells, examining the gene expression patterns and their association with prognosis and therapeutic outcomes is of paramount importance. Single-cell sequencing is a high-throughput technique that analyzes an ...