Cloning of the human IFN-β synthetic sequence and analysis of expression level in two types of vectorsThe synthetic human IFN-β structural gene was fused to synthetic ubiquitin sequence and adapted to the codon usage. We compared the stability of the same IFN-β sequence in two types of ...
Author notes A. Mikšys Present address: ATEM Structural Discovery GmbH, Remscheid, Germany L. Malinauskaitė Present address: BioNTech UK Ltd, Francis Crick Ave, Cambridge Biomedical Campus, Cambridge, UK These authors contributed equally: R. Čepaitė, N. Klein, A. Mikšys. These author...
Author notes Aviv Regev Present address: Genentech, South San Francisco, CA, USA These authors contributed equally: Karthik A. Jagadeesh, Kushal K. Dey. These authors jointly supervised this work: Alkes L. Price, Aviv Regev. Authors and Affiliations Broad Institute of MIT and Harvard, Cambridge...
DNA vaccines entail the direct, in situ inoculation of DNA-based eukaryotic expression vectors that encode the sequence of a pathogenic protein antigen. The constructed plasmids are then subsequently grown in bacteria likeE. coliand highly purified via chromatographic methods. LPS contamination of plasmid...
PCR cloning makes it possible to breed new strains of bacteria with altered genetic makeup via the insertion of amplified dsDNA fragments into the vectors such as gDNA, cDNA, and plasmid DNA. Cloning assists in introducing point mutations using site-directed mutagenesis which itself employs the re...
Recombinant adeno-associated viruses (rAAVs) are efficient gene delivery vectors via intravenous delivery; however, natural serotypes display a finite set of tropisms. To expand their utility, we evolved AAV capsids to efficiently transduce specific cell
Regarding cell tropism in vivo, VSV-G-pseudotyped SIV vectors transduced neuronal as well as glial cells, whereas all other pseudotypes preferentially transduced neuroglial cells. In addition, we analyzed the influence of the central polypurine tract (cPPT) in context of the VSV-G-pseudotyped ...
of the input to excitatory neurons as well as three inhibitory interneuron subtypes in the mPFC. On the global scale the input patterns were found to be mainly cell type independent, with quantitative differences in key brain regions, including the basal forebrain. Mapping of the local mPFC ...
Author notes These authors contributed equally: Usha K. Nivarthi, Jesica Swanstrom. Authors and Affiliations Department of Microbiology and Immunology, University of North Carolina School of Medicine, Chapel Hill, NC, USA Usha K. Nivarthi, Matthew J. Delacruz, Bhumi Patel, Ralph S. Baric & Ara...
Author notes These authors contributed equally: Usha K. Nivarthi, Jesica Swanstrom. Authors and Affiliations Department of Microbiology and Immunology, University of North Carolina School of Medicine, Chapel Hill, NC, USA Usha K. Nivarthi, Matthew J. Delacruz, Bhumi Patel, Ralph S. Baric & Ara...