In recent years, the critical role of Mx proteins in antiviral protection has been established in cell culture and animal model systems. In fishes, the antiviral property of Mx protein has also been reported. Mx has been cloned and characterized in rainbow trout,Oncorhynchus mykissWalbaum21; Atlan...
We directly cloned all ten megasynthetase gene clusters (each 10–52 kb in length) from Photorhabdus luminescens into expression vectors and expressed two of them in a heterologous host to identify the metabolites luminmycin A and luminmide A/B. We also directly cloned cDNAs and exactly defined...
We directly cloned all ten megasynthetase gene clusters (each 10–52 kb in length) from Photorhabdus luminescens into expression vectors and expressed two of them in a heterologous host to identify the metabolites luminmycin A and luminmide A/B. We also directly cloned cDNAs and exactly defined...
All the vectors were generated by Gibson assembly [24] and confirmed by Sanger sequencing and a Western blot analysis. For cloning pLV-EF1a-IRES-Blast-N-3xFLAG, the pBSCoV2-N-3xFLAG bacmid generated within this study was used as the template. All the primers used for plasmid generation ...