Protein Expression & PurificationStehling, E.G.; da Silveira, W.D.; Campos, T.A.; Brocchi, M.; Pertinhez, T.A.; Spisni, A. Development of a bacterial cloning vector for expression of scorpion toxins for biotechnological studies. Protein Expr. Purif. 2008, 57, 88-94....
The pEX plasmids are a family of expression vectors for use in . coB (1). Expression of cloned genes (as cro-lacZ fusion proteins) is controlled by the cI857 repressor gene. These vectors offer a number of dear advantages over other expression vectors; 1. Easy induction of gene expression...
Cloning expression vectors of the A. woodii F1 ATP synthase Using pKB3-His (a pET21a[+] vector derivative) as a master plasmid, which was kindly provided by Karsten Brandt (Müller laboratory27 at Goethe University), we performed PCR for cloning of deletions or point mutations and ligation ...
An expression vector of 125E11 single-chain Fv antibody fusion protein, in which VH and VL peptide were connected by a flexible linker (Gly4Ser)3, was constructed. The scFv fusion protein was highly expressed in Escherichia coli mainly in inclusion body form. Using urea and pH gradient gel...
Vector-free cloning of a bacterial endo-1,4-β-glucanase in Lactobacillus plantarum and its effect on the acidifying activity in silage: Use of recombinant... F Rossi,A Rudella,M Marzotto,... 被引量: 0发表: 2001年 Vector-free cloning of a bacterial endo-1,4-β-glucanase in ...
The functional expression of the cDNA in Escherichia coli, as an N-terminal thioredoxin fusion protein, with the pET32a vector yielding a recombinant enzyme. Sequence analysis was used to compare this enzyme with the mechanistically related epi-aristolochene synthase from tobacco, and the effect of...
Successful cloning of the insert into the vector disrupts the expression of the lethal gene and permits colony formation. The ccdB gene, derived from the F′ episome, is one of the lethal genes used for positive selection...
manipulating the bacteria to deliver a mammalian expression vector that encodes short-hairpin RNA (shRNA) [34] or by plasmid-driven expression of shRNA in the bacteria themselves [35]. The latter approach has also been demonstrated usingEscherichia coli[36]. Both approaches are reliant on tumour...
This allows the expression of antibiotic resistance gene(s) from the introduced plasmid (Figure 5). This step improves cell viability and cloning efficiency. For electroporated cells, it is recommended that the cells are grown as...
expression transgene faithfully in a copy dependent fashion (Giraldo and Montoliu, 2001; Van Keurenet al., 2009). Such large DNA fragments can be found in specialized “libraries” of genomic DNA built with special gene cloning vectors. Examples include P1 phage artificial chromosome (PAC...