Subcloning into appropriate expression vector Plasmid Prep and DNA QC Optimized gene sequence report Protein in buffered solution with your specified amount & purity QC data 2Protein Expression Evaluation Transform plasmids into appropriate bacterial expression strain ...
Enzyme for degrading acephate pesticides, coding gene, expression vector, industrial bacterial strain and application thereofThe invention discloses an enzyme for degrading acephate pesticides, coding genes, expression vectors, industrial bacterial strains and applications thereof, which is characterized in ...
To induce expression of the cloned protein, L-arabinose is added to the growth medium to express the T7 RNA polymerase, which then binds to the T7 promoter of the recombinant vector (Figure 5A). The L-arabinose concent...
C. beijerinckii is used as an expression vector of the nitroreductase gene of E. coli, which can cleave the nontoxic prodrug CB 1954 into a toxic anticancer drug. C. acetobutylicum is also used as a protein or gene delivery vector for cancer therapy. C. sporogenes is used in the enzyme ...
manipulating the bacteria to deliver a mammalian expression vector that encodes short-hairpin RNA (shRNA) [34] or by plasmid-driven expression of shRNA in the bacteria themselves [35]. The latter approach has also been demonstrated usingEscherichia coli[36]. Both approaches are reliant on tumour...
Once confirmed, desired colonies may be employed in downstream applications such as plasmid isolation, subcloning, transfection, and protein expression. Learn more about colony screening methods › Tips for successful bacterial transform...
In this method 'probe' sequences are inserted in a very small plasmid vector and introduced ... B Seed - 《Nucleic Acids Research》 被引量: 369发表: 1983年 The Impact of Recombination on dN/dS within Recently Emerged Bacterial Clones. , and Feil, E.J. ( 2011 ) The impact of ...
Then PCR-amplified DNA fragments of individual pdu genes, mCherry, and sfGFP were cloned into the linear vector by Gibson assembly103. pXG10-SF containing a constitutive PLtetO-1 promoter was employed as a backbone of expression vectors for complementation experiments104. The linear pXG10-SF was...
requiring far more clones to cover the genome than with aYAClibrary. Chimerism is not a problem with BACs, and along with P1Artificial Chromosome(PAC) libraries these are gaining in usage in sequencing projects. Because of the low copy number of the vector, large-volumepurificationsare...
rhaeticus expression vector, ori‐pBRR1 origin of replication, chloramphenicol resistance)35. K. rhaeticus cells were transformed by electroporation using 100 µl of electrocompetent cells and 20–500 µg of plasmid DNA in a 1 mm path electrocuvettes, using electroporation parameters 2.5...