Helinski DR. Plasmids as vectors for gene cloning. Basic Life Sci 1977;9:19-49.Helinski, D. 1982. Plasmids as vectors for gene cloning: Past, present, and future use. pp. 5–24 in Hollaender, A. (editor), Genetic Engineering of Microorganisms for Chemicals . Plenum Press, New York....
"Gene (or DNA) cloning" is the process of generating identical copies of a cell or an organism. Clones thus produced possess identical genes. In this technique, restriction enzymes cut DNA carrying the target gene(s) into fragments. These segments are subsequently cloned onto a cloning vector,...
recombinant viral vectors for the delivery of therapeutic genes into diseased tissues. While substantial progress has been made, and some clinical successes are over the horizon, further vector refinement and/or development is required before gene therapy will become standard care for any individual ...
in this experiment had a similarity of 100% with AtDREB2B gene logged on GenBank.Furthermore the subcloned fragment was inserted into plant expression vectors pCAMBIA1301 and pBin438 b,which were expressed by rd29A promoter and repeated 35 S promoter regulated gene separately.[Conclusion] 2 ...
In addition, for GenEZ™ ORFs cloned into custom vectors can be further customized by the addition of up to 30 bp of 5' or 3' flanking sequence – free of charge. Only$50for subcloning Choose from over150popular commercialvectors,download vector list (.xls) ...
In genetic engineering, multiple identical copies of a gene are produced in cloning vectors, such as plasmids and phages . See gene cloning. 在遗传工程上,相同的多拷贝基因是由质粒或噬菌体这样的克隆载体产生的。 dictsearch.appspot.com 2. Directional cloning vectors contain a single-strand overhang ...
gene cloning and construction of prokaryotic and plant expression vectors of ricin- a-chain/pap-s1 fusion protein and its inhibition of protein synthesis YS Hassan,SL Ogg 被引量: 0发表: 2017年 Ricin toxin contains at least three galactose-binding sites located in B chain subdomains... Studies...
Efficient cloning system for construction of gene silencing vectors in Aspergillus niger 来自 掌桥科研 喜欢 0 阅读量: 43 作者:JM Oliveira,DVD Veen,LHD Graaff,Q Ling 摘要: An approach based on Gateway recombination technology to efficiently construct silencing vectors was developed for use in the ...
13. To overcome the low efficiency, ZERO-background-cloning using theccdBgene, an active cytotoxic factor inEscherichia coli, was developed as a positive-selection system14.ccdB-sensitiveE. colicells carrying empty vectors cannot survive in this system because the expression of CcdB protein is ...
(38). Lentivirus vectors would therefore appear to be ideally suited for gene transfer to the relatively quiescent cells of the alveolar epithelium. However, the lack of a well-characterized virus receptor and the polarized nature of its distribution makes transfection efficiency in different cell ...