DNA vaccines entail the direct, in situ inoculation of DNA-based eukaryotic expression vectors that encode the sequence of a pathogenic protein antigen. The constructed plasmids are then subsequently grown in bacteria likeE. coliand highly purified via chromatographic methods. LPS contamination of plasmid...
Cloning of the human IFN-β synthetic sequence and analysis of expression level in two types of vectorsThe synthetic human IFN-β structural gene was fused to synthetic ubiquitin sequence and adapted to the codon usage. We compared the stability of the same IFN-β sequence in two types of ...
Lentiviral vectors have proven to be promising tools for transduction of brain cells in vivo and in vitro. In this study, we have examined the central nervous system (CNS) transduction efficiencies and patterns of a self-inactivating simian immunodeficiency virus (SIVmac)-derived lentiviral vector ...
This is a comparative study of AAV serotypes 1–9–mediated transgene expression after systemic delivery by TV injection. The capsid genes of the serotypes were pseudotyped by cloning into helper plasmids containing the AAV2 rep gene. The packaging vector contained the Luciferase gene cDNA driven ...
et al. Cloning of a receptor subunit required for signaling by thymic stromal lymphopoietin. Nat. Immunol. 1, 59–64 (2000). Article CAS PubMed Google Scholar Gao, P.-S. et al. Genetic variants in TSLP are associated with atopic dermatitis and eczema herpeticum. J. Allergy Clin. ...
Recombinant adeno-associated viruses (rAAVs) are efficient gene delivery vectors via intravenous delivery; however, natural serotypes display a finite set of tropisms. To expand their utility, we evolved AAV capsids to efficiently transduce specific cell
The signature feature of the midgut is its epithelium, a single cell layer acting as a barrier to separate the lumen from internal tissues. In Drosophila, the midgut epithelium comprises four principal cell types. Intestinal stem cells (ISCs) can self-renew and also give rise to progenitor cell...
Disclosed is number coding of pairs (“doublets”) or small sets (“multiplets”) of solid phase carriers which provides distinguishable subtypes of a given type of such carriers, where each carrier type is distinguishable on the bases of a different code. Such number coding is useful for augme...
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Thus, GmSNAP18 was identified to be the most probable candidate gene conferring resistance to SCN at the rhg1 locus, consistent with the results of map-based cloning (Fig. 1). The predicted GmSNAP18 protein of Forrest and Peking is different from that of PI 88788. This is consistent ...