Cloning of the human IFN-β synthetic sequence and analysis of expression level in two types of vectorsThe synthetic human IFN-β structural gene was fused to synthetic ubiquitin sequence and adapted to the codon usage. We compared the stability of the same IFN-β sequence in two types of ...
Cloning, expression, and purification of types IV-A1 and IV-A3 complexes Type IV-A1 cas genes, including a C-terminal hexa-histidine-tag encoding fusion of cas8, were cloned into the first multiple cloning site of a with EcoRV and HindIII digested pRSFDuet-1 via Gibson cloning. The same ...
Isolation, cloning and characterization of new adeno-associated virus (AAV) serotypesThe present invention provides new adeno-associated virus (AAV) viruses and vectors, and particles derived therefrom. In addition, the present invention provides methods of delivering a nucleic acid to a cell using ...
DNA vaccines entail the direct, in situ inoculation of DNA-based eukaryotic expression vectors that encode the sequence of a pathogenic protein antigen. The constructed plasmids are then subsequently grown in bacteria likeE. coliand highly purified via chromatographic methods. LPS contamination of plasmid...
These results show that spatial and temporal control of transgene expression can be achieved in rod photoreceptors. Additionally, system components are constructed in Gateway compatible vectors for the rapid cloning of doxycycline-inducible transgenes and use in other areas of zebrafish research. 展开 ...
Nuclear localization signals (NLS) are generally short peptides that act as a signal fragment that mediates the transport of proteins from the cytoplasm into the nucleus. This NLS-dependent protein recognition, a process necessary for cargo proteins to p
et al. Cloning of a receptor subunit required for signaling by thymic stromal lymphopoietin. Nat. Immunol. 1, 59–64 (2000). Article CAS PubMed Google Scholar Gao, P.-S. et al. Genetic variants in TSLP are associated with atopic dermatitis and eczema herpeticum. J. Allergy Clin. ...
Lentiviral vectors have proven to be promising tools for transduction of brain cells in vivo and in vitro. In this study, we have examined the central nervous system (CNS) transduction efficiencies and patterns of a self-inactivating simian immunodeficie
We thank the following alumni and current members of the Gradinaru group for their assistance in this study: K. Y. Chan and R. Challis for performing mouse injections, R. Hurt for performing preliminary characterization of vectors, Y. Lei for assistance with cloning, K. Beadle for vector prod...
- 《Journal of Lightwave Technology》 被引量: 95发表: 2006年 Expression vectors for hirudin, transformed cells and process for the preparation of hirudin Vector for the cloning and expression in a host cell of hirudine or an analogue of hirudine, characterized in that it comprises the gene ...