membrane of root hairs and interacts with the tip-growth regulator ARMADILLO REPEAT ONLY PROTEINs (AROs)26,27, implying its potential function as a local inactivator. In Arabidopsis, only two RopGAPs are detectable in pollen tubes. However, their loss of function does not cause discernable growth...
The efficient folding of proteins into their three-dimensional functional structure is of fundamental importance for cell viability. In vitro studies of protein folding have provided detailed insights into the folding process by offering precise control of experimental conditions1,2. To date, most foldin...
Water-soluble membrane proteins may be analyzed by a new, rapid technique that combines electrophoresis on high-resolution sodium dodecyl sulfate (SDS) polyacrylamide gels and immunoelectrophoresis. After separation in the first dimension by electrophoresis in SDS, the proteins are subjected to a second...
The cellular localization of these proteins has been studied in Nostoc sp. PCC 7120, where the MnSod is membrane bound and the FeSod is cytosolic (Li et al., 2002; Regelsberger et al., 2004). This different localization could be responsible for different roles in protecting the cells ...
They consist of six transmembrane domains and two NPA motifs. This constitutes two pores in the protein structure, which are essential for the water movement across the cell membrane (Wudick et al., 2009). Table 1. Aquaporins differentially expressed (RNA and the correspondent proteins) in E....
Measuring only 5 nm in diameter, microfilaments are about the same thickness as the cell membrane. Found throughout the neuron, they are particularly numerous in the neurites. Microfilaments are braids of two thin strands that are polymers of the protein actin. Actin is one of the most abund...
These results suggest that it may be feasible to use multilayer assemblies of functional proteins and enzymesin high-temperature applications. 展开 关键词: Stabilization of the membrane protein bacteriorhodopsin to 140 ...C in two-dimensional films, Artículo ...
These alkali-labile phosphoproteins can be separated by electrophoresis in acid medium. The 130 kDa phosphoprotein resembles a corresponding protein in the erythrocyte membrane, whereas the 100 kDa protein resembles that of the Ca2+-transport ATPase in sarcoplasmic reticulum from skeletal muscle. These ...
3h). Pathway analysis indicated that CREB3L1 was involved in co-translational protein targeting to membrane, the establishment of protein localization to ER, and actin filament reorganization (Fig. 3i). Together, these results reveal the distinct transcriptional profiles of S100P + SPP1− ...
These domains are fused to a protein of interest, to generate two “hybrid” proteins, which are subsequently transformed in to a compatible yeast strain. Ideally, when the two proteins interact, the reconstituted transcription activator promotes the expression of a reporter gene, which then confers...