Surprisingly, the genetic complementation studies did not restore the 3xD4 STM strain to wild-type levels of cyst formation in vivo. Similarly, disruption of the 3xD4 locus in a wild-type background did not result in decreased lethality and cyst burden compared to wild-type in vivo. Current...
Two-dimensional crystallization of membrane proteins: a practical guide. In: Membrane Protein PuriWcation and Crystallization: A Practical Approach, second ed. Academic Press, San Diego, pp. 253-284.Kuhlbrandt, W. (2003) Two-dimensional crystallization of membrane proteins: a practical guide in: ...
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Membrane protein research has flourished in the past 20 years. Exciting technological innovations in structural biology, including cryoEM single-particle analysis and AI-based protein structure prediction, such as AlphaFold 2, have largely revolutio
Two-dimensional (2D) crystallisation of Membrane proteins reconstitutes them into their native environment, the lipid bilayer. Electron crystallography allows the structural analysis of these regular protein–lipid arrays up to atomic resolution. The crystal quality depends on the protein purity, ist stab...
a trimeric integral membrane glycoprotein. Another gene of potential biological relevance at the chromosome 8 locus is the tumour suppressor candidate 3 (TUSC3), which encodes a protein localised to the endoplasmic reticulum and acts as a component of the oligosaccharyltransferase complex, responsible...
Enhanced prezygotic isolation in sympatry has become a well-known feature of gamete recognition proteins in marine broadcast spawners. But in most cases the evolutionary process or processes responsible for this pattern have not been identified. Although gamete recognition proteins inC. intestinalisdo appe...
However, the cell wall composition was not of significance in protein export and the parameters for the excretion process were found to be environmental. In particular, two types of extracellular proteins were identified: the first type of enzyme, penicillinase, was only weakly catabolite repressed;...
ACE2 is a membrane-bound protein with the highest levels of transcripts in the lungs, the gastrointestinal and renal systems, and all cardiac tissues. Both membrane-bound and soluble ACE2 are parts of the renin–angiotensin–aldosterone system (RAAS), and ACE2 is also involved in the breakdown...
Preparation of membrane proteins for analysis by two-dimensional gel electrophoresis[J].Journal of Chromatography B:Biomedical Sciences and Applications,2006.Kashino Y, Harayama T, Pakrasi HB, Satoh K (2007) Preparation of membrane proteins for analysis by two-dimensional gel electrophoresis. J ...