Walker, Identification of protective components of two major outer membrane proteins of spotted fever group rickettsiae, Am. J. Trop. Med. Hyg. 65 (2001) 371e378.Diaz-Montero, C.M., Feng, H.M., Crocquet-Valdes, P.A. and Walker, D.H. (2001) Identification of protective components of...
Two-dimensional crystallization of membrane proteins: a practical guide. In: Membrane Protein PuriWcation and Crystallization: A Practical Approach, second ed. Academic Press, San Diego, pp. 253-284.Kuhlbrandt, W. (2003) Two-dimensional crystallization of membrane proteins: a practical guide in: ...
Membrane protein research has flourished in the past 20 years. Exciting technological innovations in structural biology, including cryoEM single-particle analysis and AI-based protein structure prediction, such as AlphaFold 2, have largely revolutio
TMA tissue microarray. Part of the picture was adapted from motifolio.com.bHeatmap showing the expression of marker genes in the indicated cell types.cChromosomal landscape of inferred large-scale copy number variations (CNVs) in nonmalignant epithelial cells (top) and malignant cells from 14 iCCA...
a trimeric integral membrane glycoprotein. Another gene of potential biological relevance at the chromosome 8 locus is the tumour suppressor candidate 3 (TUSC3), which encodes a protein localised to the endoplasmic reticulum and acts as a component of the oligosaccharyltransferase complex, responsible...
Using high-throughput multiplex immunoassays, 276 cardiometabolic plasma proteins were measured in two subcohorts. We applied multivariable-adjusted regression models using a discovery-replication design to examine protein associations with increasing consumption of non-fermented or fermented milk. Results The...
Two-dimensional (2D) crystallisation of Membrane proteins reconstitutes them into their native environment, the lipid bilayer. Electron crystallography allows the structural analysis of these regular protein–lipid arrays up to atomic resolution. The crystal quality depends on the protein purity, ist stab...
CPPs are generally short peptides consisting of < 30 amino acids and are divided into two main types as follows: 1) a polypeptide motif derived from natural proteins with penetrating functions, and 2) artificially designed and synthesized polypeptides, which are further optimized as molecule-interna...
Lack of detection of interactions for certain proteins. Proteins that cannot fold intracellularly (e.g., membrane proteins) or cannot enter the nucleus cannot be studied using transcription-based assays. Protein fusions that are either toxic to or unstable in yeast (Y2H) orE. coli(B2H) cannot ...
Leptin-deficient ob/ob mice and leptin receptor-deficient db/db mice are commonly used mice models mimicking the conditions of obesity and type 2 diabetes development. However, although ob/ob and db/db mice are similarly gaining weight and developing mas