b The pie chart shows the proportion of cell groups in the tumor ecosystem and the population size of each cell group. c The heatmap shows the marker genes and their expression patterns in identified immune cells, malignant cells, and stromal cells. d A schematic description of tumor ...
Table S1. Proportion test of significant bacteria between normal breast and breast tumor, related to Figure 1 Table S2. Proportion test of significant bacteria between tumor tissue and tumor cell, related to Figure 2 Table S3. Four bacterial clusters in normal breast, breast tumor, met adjacent...
Cell type was annotated using known marker genes34,35,69,70,71. Tumor cells were identified based on the inferred presence of somatic CNAs by inferCNV72. We further merged Seurat68-identified clusters that were not significantly different in gene counts, which is the total number of expressed ...
Figure 1. Identification of MARCO as a Marker on M2 Macrophages in Tumor Stroma of Mammary Carcinoma, Melanoma, and Colon Carcinoma (A) Immunofluorescence (IF) staining of 4T1 mammary tumor sections. MARCO (red) as an individual channel (left) and merged (right) with CD11b (green) and F4...
www.nature.com/scientificreports OPEN Circulating Tumor Cell Count Correlates with Colorectal Neoplasm Progression and Is a received: 09 October 2015 Prognostic Marker for Distantaccepted:31March2016 Published:14April2016 Metastasis in Non-Metastatic Patients Wen-Sy Tsai1,2, Jinn-Shiun Chen1,2, ...
Remarkably, CK18-positive CTCs were detected in two NSCLC patients, with a tumor marker-positive detection rate of 4.2% (2/48). The circulating tumor microemboli (CTM) were detected in 4 lung cancer patients, while not in the other groups. Similarly, CTECs were detected in almost all ...
We then extracted the subtype 1 specific marker genes (monocytes) to conduct a KEGG enrichment analysis, revealing that these monocytic biomarkers mainly participate in the regulation of several immunological pathways, including the positive regulation of the inflammatory response (Figure S5H). These ...
The corrected PC matrixes were used to perform unsupervised shared-nearest neighbor–based clustering and UMAP visualization analysis. Differential expression analysis based on clusters was used to identify marker genes and manually annotate clusters based on information from literature....
AWorkflow of patient sample screening, single-cell sequencing, spatial transcriptomics and combined analysis.BMain cell types and tumor cell marker validation and T-cell subsets in TME of PCNSL patients. Full size image Section fixation, staining and imaging ...
Tumor infiltrating lymphocytes (TILs) in GBM have been shown to express PD-1, a marker of exhaustion, at higher rates than peripheral T cells in the bloodstream [8]. Glioma-associated IL-10 upregulates the expression of PD-L1 on TAMs and peripheral monocytes, which can then bind to and ...