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1.tRNAprocessinginprokaryotes •大肠杆菌tRNA操纵子中包含tRNA的编码区域；•还包括多至7种tRNA基因的操纵子 MaturetRNAsaregeneratedbyprocessinglongerpre-tRNAtranscripts,whichinvolves1.specificexo-andendo-nucleolyticcleavagebyRNasesD,E,FandP(general)followedby2.basemodificationswhichareuniquetoeachparticulartRNA...
9月28日,上海交通大学医学院附属第九人民医院上海精准医学研究院雷鸣研究员、武健研究员联合中科院大连化学物理研究所李国辉研究员在Science杂志发表了题为 “Structural insight into precursor tRNA processing by yeast ribonuclease P”的研究长文,该研究首次揭示了真核生物中tRNA前体5’端的加工成熟机制。
ribonucleasesRNase PPRORPNYNVapCTransfer RNAs require essential maturation steps to become functional. Among them, RNase P removes 5' leader sequences of pre-tRNAs. Although RNase P was long thought to occur universally as ribonucleoproteins, different types of protein-only RNase P enzymes were ...
相关研究结果发表在2018年11月9日的Science期刊上,论文标题为“Structural insight into precursor tRNA processing by yeast ribonuclease P”。 这种酵母RNase P全酶由一个具有催化能力的RNA (即Rpr1)和9个蛋白组分组成。Rpr1 RNA采取一种延伸的单层构象。这种单层构象维持一种中央螺旋核心,但缺乏大多数让细菌RNase...
来自上海交通大学附属第九医院精准医学研究院,中科院大连化物所发表了题为“Structural insight into precursor tRNA processing by yeast ribonuclease P”的文章,成功解析了酵母内源RNase P 全酶及其与底物pre-tRNA的复合物结构,这一里程碑式的研究工作首次完整的提出了真核生物RNase P催化底物tRNA前体切割成熟的分子机...
[5] SEKULOVSKI S, DEVANT P, PANIZZA S, et al. Assembly defects of human tRNA splicing endonuclease contribute to impaired pre-tRNA processing in pontocerebellar hypoplasia [J]. Nat Commun, 2021, 12(1): 5610. 来源/...
Spatial/temporal control of Cas9 guide RNA expression could considerably expand the utility of CRISPR-based technologies. Current approaches based on tRNA processing offer a promising strategy but suffer from high background. Here, to address this limita